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改善作物的光合性能能够有效突破作物的产量潜力,目前国内关于转高粱C4型pepc基因大豆的研究尚未见报道。本研究以东北地区主栽大豆品种‘黑农41’为材料,通过农杆菌介导法将高粱C4型全长pepc基因转入‘黑农41’,获得了转基因植株。通过对转基因植株T0代和T1代进行了分子生物学检测、农艺性状调查和生理指标检测,结果表明目的基因已整合到大豆基因组中,T0代和T1代植株的净光合速率、气孔导度和蒸腾速率均有不同程度的提高,而胞间CO2浓度降低,其中T0代和T1代植株的净光合速率分别比对照提高了37.4%和20.7%,在T1代净光合速率增幅最大的T1-3-3,单株产量增加也最多,说明pepc基因的导入改善了转基因植株的光合特性,进而使转基因大豆产量提高。本研究可为高光效转基因大豆新品种的选育提供重要素材。
Improving the photosynthetic characteristics of crops can effectively break the yield potential of crops. At present, there is no report on the research about the transgenic peas C4 soybean. In this study, the main soybean cultivar ’Heinong 41’ in northeast China was used as material, and the full-length pepc gene of C4 type of sorghum was transferred into ’Heinong 41’ by Agrobacterium-mediated method. Transgenic plants were obtained. The results of molecular biology, agronomic traits and physiological tests of T0 generation and T1 generation of transgenic plants showed that the target gene was integrated into soybean genome. The net photosynthetic rate, stomatal conductance, (P <0.05). The net photosynthetic rate of T0 generation and T1 generation plants increased by 37.4% and 20.7%, respectively, compared with the control, respectively. In T1-3, the highest increase of net photosynthetic rate -3, the highest yield per plant, indicating that the introduction of pepc gene improved the photosynthetic characteristics of transgenic plants, thereby increasing the yield of transgenic soybean. This study can provide important material for the selection and breeding of high photosynthetic efficiency transgenic soybean.