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环卵沉淀反应是血吸虫病免疫诊断中较普遍应用的一种方法,具有较高的敏感性和特异性。减压冰冻干燥虫卵的制备,解决了虫卵抗原的保存和供应问题。作者在用1.5%甲醛处理鲜卵解决了干卵的卵壳破裂问题以后,本文进一步对其保存期限、敏感性、特异性、疗效考核及现场查病价值等作了观察,结果如下: 干卵在室温保存3个月直至3年,作环卵沉淀反应,环沉率与保存前基本相似,但保存3个月后(+++)反应逐渐下降。对粪检阳性的血吸虫病患者1,022例做干卵环卵反应,平均阳性率为97.3%,其中环沉率>5%者占阳性反应者的81.9%。对109只感染4～800条尾蚴的不同感染度的家兔做干卵环卵反应均呈阳性。对993例正常人做干卵环卵反应,假阳性率为3.1%,但出现反应的31例中仅1例环沉率超过5%,且假阳性反应沉淀物的形态大都为小泡状或球状。对丝虫病、疟疾、华枝睾吸虫病和囊虫病共62例作干卵环卵反应,仅1例丝虫病出现阳性,环沉率为1%。说明干卵环卵沉淀反应有较高的敏感性和特异性。
Ovorrhea and egg precipitation reaction is a more commonly used method of immune diagnosis of schistosomiasis, with high sensitivity and specificity. Decompression freeze-dried eggs preparation, to solve the preservation and supply of egg antigen. After the treatment of fresh eggs with 1.5% formaldehyde to solve the problem of eggshell rupture of dry eggs, the author further observed the shelf-life, sensitivity, specificity, curative effect and the value of spot check, the results are as follows: After 3 months of storage at room temperature until 3 years, as a ring egg precipitation reaction, the recovery rate was similar to before storage, but after 3 months of storage (+++) the reaction decreased gradually. Ovarian ovo reaction was performed on 1,022 patients with positive schistosomiasis, with an average positive rate of 97.3%, of which, the rate of cysts> 5% was 81.9% of the positive ones. 109 rabbits infected with 4-800 cercariae with different infection degrees were all oval eggs. For the 993 cases of normal people oval egg reaction, the false positive rate was 3.1%, but the response of 31 cases, only 1 case of ring deposition rate of more than 5%, and most of the false positive reaction precipitate vesicles or Globular. 62 cases of filariasis, malaria, clonorchiasis and cysticercosis were oval eggs reaction, only 1 case of filariasis positive, loop Shen rate of 1%. Description oval egg precipitation reaction has a high sensitivity and specificity.