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目的探讨Orexin A对抑郁症模型大鼠的学习记忆能力的影响。方法实验大鼠随机分为空白组、空白对照组、抑郁对照组、抑郁治疗组。除空白组,其余3组实验前施以杏仁体埋管手术。抑郁对照组、抑郁治疗组采用孤养加慢性不可预见性的温和应激(Chronic Unpredictable Mild Stress,CUMS)诱导抑郁症大鼠模型,分别采用旷场实验、糖水偏爱百分比及体重及摄食量测定对抑郁大鼠模型评分。空白组和空白对照组不注射任何试剂,抑郁治疗组给Orexin A,抑郁对照组给等量生理盐水,采用平衡木和Morris水迷宫测试记录大鼠学习记忆成绩。结果空白对照组的水平得分、垂直得分、糖水偏爱百分比、体重和摄食量与空白组相比,差异均无统计学意义(P>0.05);而抑郁对照组和抑郁治疗组的水平得分、垂直得分、糖水偏爱百分比、体重和摄食量与空白对照组相比,差异均有统计学意义(P<0.05);抑郁治疗组的平衡木和Morris水迷宫测试结果均短于抑郁对照组,差异有统计学意义(P<0.05)。结论采用孤养加CUMS可建造大鼠抑郁症模型,Orexin A能够改善抑郁症模型大鼠的学习记忆能力。
Objective To investigate the effect of Orexin A on the learning and memory abilities of depression model rats. Methods Experimental rats were randomly divided into blank group, blank control group, depression control group and depression treatment group. In addition to the blank group, the other three groups were implanted with amygdala before surgery. Depression control group and depression treatment group were induced by deprivation and chronic unpredictable Mild Stress (CUMS), and the open field test, percentage of sweet and sour water preference and body weight and food intake were used respectively Depression rat model score. The blank group and the blank control group were not injected with any agent, depression treatment group to Orexin A, depression control group to give the same amount of saline, the balance beam and Morris water maze test record learning and memory in rats. Results There was no significant difference between the control group and the blank group (P> 0.05). However, the scores of horizontal score, vertical score, percentage of sweet water preference, body weight and food intake of blank control group were not significantly different from those of blank control group Score, percentage of sugar preference, body weight and food intake compared with the blank control group, the difference was statistically significant (P <0.05); depression treatment group, the balance beam and Morris water maze test results were shorter than the depression control group, the difference was statistically Significance (P <0.05). Conclusion Occlusion and CUMS can be used to establish a model of depression in rats. Orexin A can improve the learning and memory ability of depression model rats.