论文部分内容阅读
目的了解深圳市龙岗区幼儿轮状病毒(Rotavirus,RV)腹泻发病情况及分子流行病学特征,为实施RV免疫规划提供依据。方法分析2009年深圳市龙岗区疫情监测系统中5岁以下婴幼儿感染性腹泻的发病情况,用酶联免疫吸附试验(ELISA)检测腹泻患儿粪便中RV,然后采用逆转录-聚合酶链反应(RT-PCR)方法对提取的RV-RNA做基因分型。结果 2009年调查地区各级监测医院共报告5岁以下感染性腹泻儿童5893例,其中RV阳性2 303例,阳性率为39.1%。随着年龄增大,儿童RV阳性率逐渐减小,且差别有统计学意义(P<0.01)。1月~(41.8%)和10月~(43.9%)两个季节腹泻儿童RV阳性率高于4月~(30.8%)和7月~(26.8%)(P<0.01)。男性与女性患儿RV检出率高于女性患儿,差异有统计学意义(P<0.05)。在随机抽取100份经ELISA检测RV阳性的2303份标本中,RT-PCR法进行轮状病毒基因G、P分型,分型率为64.0%,其中P8G3为主要流行型,占所有分型的42.0%。结论 RV是深圳市婴幼儿秋冬季腹泻最主要的病原体,以G3型为主,可作为研究RV疫苗的主要血清型。
Objective To understand the incidence and molecular epidemiological characteristics of rotavirus (RV) diarrhea in Longgang District of Shenzhen City and provide the basis for the implementation of RV immunization program. Methods The incidence of infectious diarrhea in infants under 5 years of age in 2009 in Longgang District of Shenzhen City was monitored. RV in feces of children with diarrhea was detected by enzyme - linked immunosorbent assay (ELISA), and then reverse transcriptase - polymerase chain reaction (RT-PCR) method to genotype the extracted RV-RNA. Results A total of 5893 cases of infectious diarrhea children under 5 years of age were surveyed at all monitoring hospitals in the survey area in 2009, of which 2 303 were positive for RV, the positive rate was 39.1%. With the increase of age, the positive rate of RV in children gradually decreased, and the difference was statistically significant (P <0.01). The positive rate of RV in children with diarrhea from January to April (41.8%) and from October to (43.9%) was higher than that from April to 30.8% and from July to 26.8%, respectively (P <0.01). The detection rate of RV in males and females was higher than that in females, the difference was statistically significant (P <0.05). Among 2303 specimens positive for RV detected by ELISA, the genotypes of rotavirus G and P were detected by RT-PCR. The typing rate was 64.0%, of which P8G3 was the predominant type, accounting for all genotypes 42.0%. Conclusions RV is the most important pathogen of infant diarrhea in infants and young children in Shenzhen City. G3 is the predominant pathogen and can be used as the main serotype in the study of RV vaccine.