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实验所用巴西盾柱木种子由圣保罗州Anhembi公司提供,种子经由98%的浓硫酸预处理15 min, 以打破物理休眠。分别在0.2%克菌丹溶液10 oC和27 oC下,-1.0 MPa聚乙二醇6000 10 oC和27 oC下,以及, 0.5 mol,0.75 mol,1.0 mol 硝酸钾溶液进行引物处理. 包括对照组种子,共8个实验处理,每一个处理5个重复,每一个处理实验用100粒种子,置于滤纸浸透引物聚乙二醇6000溶液的15厘米培养皿中,置于冰箱保持27oC恒温。实验引物渗透势为0.0, -0.2, -0.4, -0.6, -0.8, -1.0-MPa, -1.4 MPa. 实验同时将种子置于浸有引物聚乙二醇的卷纸中,观察水胁迫下的萌发情况。实验结果证明,随着聚乙二醇6000浓度的升高,巴西盾柱木种子萌发率降低,对照组萌发率比实验引物处理组高。聚乙二醇达到PEG -1.4 MPa,种子不能萌发。图2表3参10。
Experiments using Brazil shield seeds provided by Anhembi, São Paulo State, the seeds were pretreated with 98% concentrated sulfuric acid for 15 min to break the physical dormancy. The primers were treated with 0.2% Captodin at 10 oC and 27 oC, -1.0 MPa PEG 6000 at 10 oC and 27 oC, and 0.5 mol, 0.75 mol and 1.0 mol potassium nitrate solution respectively. The control group Seeds, a total of eight experimental treatments, each dealing with five replicates, each processing experiment with 100 seeds, placed in filter paper impregnated with polyethylene glycol 6000 solution 15 cm petri dish, placed in the refrigerator to maintain 27oC constant temperature. The osmotic potentials of the experimental primers were 0.0, -0.2, -0.4, -0.6, -0.8, -1.0-MPa, -1.4 MPa. At the same time, the seeds were placed in a paper roll impregnated with the primer polyethylene glycol, and observed under water stress Germination. The experimental results showed that the seed germination rate of C. pilosicoli decreased with the increase of the concentration of PEG 6000, and the germination rate of the control group was higher than that of the experimental group. PEG reaches PEG -1.4 MPa, seeds can not germinate. Figure 2 Table 3 Reference 10.