目的了解噻唑橙(TO)在红细胞保养液MAP液中的吸收光谱特性及其稳定性;了解红细胞(RBC)对TO吸附吸收、洗脱的动态过程。方法用MAP液配制120 mmol/L TO-MAP液,4℃避光保存,于配制后d 0、3、17,分别用可见紫外分光光度计进行350～600 nm扫描,观察其吸收光谱的变化;配制成TO-MAP-RBC悬液,于第0、2、4、12、24 h分别离心,取上清液测定其350～600 nm的扫描光谱;取储存2 d的TO-MAP-RBC液,离心弃上清,加生理盐水洗涤6次,每次间隔20 min,收集每次洗涤液,测定其350～600 nm扫描光谱。结果 MAP液中TO吸收主峰位于468 nm,在430 nm和520 nm有2处肩峰,储存0、7 d TO-MAP液扫描线几乎完全重叠,储存17 d扫描线略有降低;TO-MAP-RBC悬液0、2、4、12、24 h其上清液的扫描线有较大的降低;洗涤液的扫描光谱显示,第1～2次洗涤TO被洗脱的量较大,第3～6次洗涤TO被洗脱的量较小,并趋于平稳。结论 TO在MAP液中非常稳定,这为TO光化学病原体灭活提供了必要条件;TO可被红细胞吸附或吸收;TO洗脱的动力学过程提示,为降低TO对测定红细胞活性功能指标的影响,可增加洗涤次数及延长洗涤间隔。 OBJECTIVE To investigate the absorption spectrum and stability of thiazole orange (TO) in the erythrocyte maintenance solution (MAP) solution and to understand the dynamic process of absorption and elution of erythrocytes (RBC) to TO. Methods 120 mmol / L TO-MAP solution was prepared by MAP solution and stored in the dark at 4 ℃. The absorbance spectra were observed by visible ultraviolet spectrophotometer at 350-600 nm on day 0, . The TO-MAP-RBC suspension was prepared and centrifuged at 0, 2, 4, 12 and 24 h respectively. The supernatant was used to measure the spectrum of 350-600 nm. TO-MAP-RBC The solution was centrifuged, the supernatant was centrifuged, washed 6 times with saline solution, and separated by 20 min intervals. Each wash solution was collected and its scanning spectrum was measured at 350-600 nm. Results The main peak of TO absorption in MAP solution was located at 468 nm. There were two shoulder peaks at 430 nm and 520 nm. The scan lines of TO-MAP solution stored for 0 and 7 d were almost completely overlapped and the scan line stored for 17 d decreased slightly. TO- -RBC suspension 0,2,4,12,24 h scan line of the supernatant has a greater reduction; washing liquid scanning spectra showed that the first to the second wash was eluted TO large amount of the first The amount of eluted TO was smaller in 3 to 6 washings and tended to be steady. Conclusion TO is very stable in MAP solution, which provides the necessary conditions for the inactivation of TO photochemical pathogens. TO can be adsorbed or absorbed by erythrocytes. The kinetics of TO elution indicates that in order to reduce the influence of TO on the determination of erythrocyte activity, Can increase the number of washing and extend the washing interval.