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从太平洋西加罗林群岛的帕劳(Palan)岛、莱恩群岛的范林(Fanning)岛、马歇尔群岛和夏威夷群岛采集了107种海藻,经冷冻或风干保存,用二种方法进行提取, 方法Ⅰ:在Waring匀浆器中,加入比样品重量5～10倍的有机溶剂,匀浆5分钟,混合物放置24小时或搅拌4～5小时,离心,上清液经真空蒸发,得粗脂溶性提取物。沉淀的残渣经干燥后用5～10倍量的水浸泡并搅拌4～5小时,放置24小时或更长的时间,离心,上清液冰冻干燥,得到水溶性提取物。水溶性提取物进行凝胶过滤(葡聚糖凝胶G-25),用0.1M碳酸氢铵洗脱,然后用阳离子交换层析(CM-葡聚糖凝胶C-25),活性成份以0.03～3.0M的醋酸铵梯度洗脱,于280nm处用紫外监测柱层析,得到初步纯化的流份。
107 species of seaweeds were collected from Palan Island, Western Caroline Islands, Fanning Island, Marshall Islands and Hawaiian Islands of the Pacific Islands, frozen and air-dried, and extracted by two methods I: In a Waring homogenizer, add 5 to 10 times the weight of the sample organic solvent, homogenized for 5 minutes, the mixture was left for 24 hours or stirred for 4 to 5 hours, centrifuged, the supernatant was evaporated in vacuo to give crude fat-soluble Extract. The precipitated residue is soaked in 5 to 10 times amount of water after being dried and stirred for 4 to 5 hours, left for 24 hours or longer, centrifuged, and the supernatant is lyophilized to obtain a water-soluble extract. The water-soluble extract was subjected to gel filtration (Sephadex G-25), eluted with 0.1 M ammonium bicarbonate, and then purified by cation exchange chromatography (CM-Sephadex C-25) 0.03 ~ 3.0M ammonium acetate gradient elution at 280nm with UV monitoring column chromatography to obtain a preliminary purification of the flow.