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【目的】探讨血小板GPⅡb/Ⅲa受体拮抗剂RGDS对血小板聚集和血小板[Ca2+]i的影响。【方法】比浊法测定PAG;采用Fura-3/AM荧光探针标记血小板胞浆钙离子,使用共聚焦显微镜观察单个血小板荧光强度的变化,计算机图像系统分析血小板[Ca2+]i的变化。【结果】凝血酶(0.03U/mL)为激动剂时,血小板PAG(M)为(78.2±12.4)%。在所取的62.5～1000μmol/LRGDS内的5种浓度下,RGDS可呈浓度依赖性地抑制凝血酶诱导的PAG(M)。正常人静息血小板[Ca2+]i荧光强度值为376.1±70.5;凝血酶(0.03U/mL)激动的血小板[Ca2+]i荧光强度值升高为977.9±108.8;GPⅡb/Ⅲa受体拮抗剂RGDS(250μmol/L)对静息血小板的[Ca2+]i荧光强度值无抑制作用;GPⅡb/Ⅲa受体拮抗剂RGDS(250μmol/L)作用于血小板后,凝血酶(0.03U/mL)激动的血小板[Ca2+]i荧光强度值升高受到抑制,抑制率为(9.37±7.5)%。【结论】凝血酶(0.03U/mL)可以引起血小板的聚集和血小板[Ca2+]i的升高。GPⅡb/Ⅲa受体拮抗剂RGDS可以抑制凝血酶(0.03U/mL)引起的血小板聚集和[Ca2+]i的升高。
【Objective】 To investigate the effect of platelet GPⅡb / Ⅲa receptor antagonist RGDS on platelet aggregation and platelet [Ca2 +] i. 【Method】 Neutrophil assay was used to measure PAG. Fura-3 / AM fluorescent probe was used to label platelet cytoplasmic Ca2 +. Confocal microscopy was used to observe the change of fluorescence intensity of single platelet. The change of platelet [Ca2 +] i was analyzed by computer image system. 【Results】 The platelet PAG (M) was (78.2 ± 12.4)% when thrombin (0.03U / mL) was used as agonist. RGDS inhibited thrombin-induced PAG (M) in a concentration-dependent manner at five concentrations of 62.5 ~ 1000μmol / LRGDS. Fluorescence intensity of platelet [Ca2 +] i resting in normal subjects was 376.1 ± 70.5; the fluorescence intensity of platelet [Ca2 +] i stimulated by thrombin (0.03U / mL) was increased to 977.9 ± 108.8; GPⅡb / Ⅲa receptor antagonist RGDS (250μmol / L) did not inhibit the fluorescence intensity of [Ca2 +] i in resting platelets; GPIIb / Ⅲa receptor antagonist RGDS (250μmol / L) The increase of [Ca2 +] i fluorescence intensity was inhibited and the inhibition rate was (9.37 ± 7.5)%. 【Conclusion】 Thrombin (0.03U / mL) can cause platelet aggregation and increase of platelet [Ca2 +] i. GPⅡb / Ⅲa receptor antagonist RGDS can inhibit thrombin (0.03U / mL) induced platelet aggregation and [Ca2 +] i increased.