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[Objective]The aim was to isolate and identify an endophytic bacteria strain with antimicrobial activity from Davidia involucrate Brail.[Method]Endophytic strain with antibiosis ability was isolated from D.involucrate Brail by using cylinder-plate method.Then,it was identified through physiological and biochemical tests,16S rDNA homology analysis as well as some gene-specific sequence analysis.[Result]B221 stain had antimicrobial activity against a variety of rice plant pathogens,and it was identified as Bacillus subtilis.[Conclusion]This study enriches the research on endophyte within D.involucrate Brail,application of Bacillus bio-control,and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens.
[Objective] The aim was to isolate and identify an endophytic strain strain with antimicrobial activity from Davidia involucrate Brail. [Method] Endophytic strain with antibiosis ability was isolated from D. involucrate Brail by using cylinder-plate method. physiological and biochemical tests, 16S rDNA homology analysis as well as some gene-specific sequence analysis. [Result] B221 stain had antimicrobial activity against a variety of rice plant pathogens, and it was identified as Bacillus subtilis. [Conclusion] This study enriches the research on endophyte within D. involucrate Brail, application of Bacillus bio-control, and therefore has laid a good foundation for the development of fungus used in biological control of crop pathogens.