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目的：研究小鼠重组Ｐｅｒｉｏｓｔｉｎ对人牙周膜细胞（ＰＤＬ细胞）在正常及病变牙根片上附着和增殖的影响。方法：将正常及病变根片分别用Ｐｅｒｉｏｓｔｉｎ、ＦＮ和无血清ＤＭＥＭ处理后，观察ＰＤＬ细胞在其上２４ ｈ的附着量和７２ ｈ增殖量，并在扫描电镜下观察其形态变化。结果：Ｐｅｒｉｏｓｔｉｎ与阳性对照 ＦＮ均可有效促进ＰＤＬ细胞在正常及病变根面上的附着和增殖（Ｐ＜０．０５～０．０１），二者促附着作用相近（Ｐ＞０．０５），但在促增殖方面，Ｐｅｒｉｏｓｔｉｎ较ＦＮ作用明显要弱（Ｐ＜０．０５）。结论：小鼠重组Ｐｅｒｉｏｓｔｉｎ对ＰＤＬ细胞在正常及病变根片上的附着和增殖均有一定促进作用。
Objective: To investigate the effect of recombinant Periostin on the attachment and proliferation of human periodontal ligament cells (PDL cells) on normal and diseased root discs. Methods: The normal and diseased root slices were treated with Periostin, FN and serum-free DMEM respectively. The attachment of PDL cells for 24 h and the proliferation for 72 h were observed. The morphology of PDL cells was observed under scanning electron microscope. Results: Both Periostin and positive control FN could effectively promote the adhesion and proliferation of PDL cells on the normal and diseased root surfaces (P <0.05 ~ 0.01) However, Periostin was significantly weaker than FN in promoting proliferation (P <0.05). Conclusion: The mouse recombinant Periostin can promote the attachment and proliferation of PDL cells in normal and diseased root slices.