论文部分内容阅读
目的:探讨生物波调控因子(BRF)对实验性脑梗死大鼠脑组织IL-6表达的影响。方法:成年健康雄性SD大鼠90只随机分为BRF组、生理盐水组和假手术组。制备大脑中动脉梗死(MCAO)模型,术后1h以1ml/100g的剂量分别腹腔注射1.25%BRF溶液和生理盐水,此后1次/d。进行行为学评分、测定脑组织含水量、HE染色观察组织病理学改变、免疫组织化学方法测定脑组织IL-6的动态变化。结果:生理盐水组及BRF治疗组脑梗死组织周围组织含水量于24h开始明显升高,48h达高峰,持续至72h,7d时明显下降,在各个时间点与假手术组比较均有统计学意义。除假手术组外,其余各组梗死区炎细胞浸润和IL-6阳性细胞表达于梗死后6h开始增多,48h达高峰,并持续至7d;假手术组未见明显炎细胞浸润,可见少量IL-6阳性细胞。BRF治疗组大鼠行为学评分降低,脑组织水肿程度减轻,术后48h最显著,病理损伤减轻,脑组织IL-6阳性细胞减少,术后48h、72h最显著。结论:生物波调控因子可以通过减轻梗死后脑水肿,降低脑内IL-6的表达,从而对大鼠缺血性脑组织损伤产生保护作用。
Objective: To investigate the effect of bio-wave factor (BRF) on the expression of IL-6 in brain tissue of experimental cerebral infarction rats. Methods: Ninety adult male Sprague-Dawley rats were randomly divided into BRF group, saline group and sham operation group. Middle cerebral artery occlusion (MCAO) model was prepared. The rats were injected intraperitoneally with 1.25% BRF solution and normal saline at a dose of 1 ml / 100 g 1 h after operation and once a day thereafter. Behavioral score, determination of brain water content, histological changes were observed by HE staining, immunohistochemical determination of brain tissue IL-6 dynamic changes. Results: The water content of the tissue around the cerebral infarction in the saline group and the BRF treatment group increased significantly from 24h, reached the peak at 48h, and continued to 72h, 7d significantly decreased, at each time point compared with the sham operation group were statistically significant . Except the sham-operated group, inflammatory cell infiltration and expression of IL-6 positive cells in the infarction area of all the other groups began to increase at 6h after infarction, reached peak at 48h and lasted for 7 days. There was no obvious inflammatory cell infiltration in the sham operation group, -6 positive cells. The scores of behavioral score decreased, the edema degree of brain tissue decreased in BRF treatment group, the most obvious was 48h after operation, the pathological damage was alleviated, and the IL-6 positive cells in brain tissue were decreased. The most significant was at 48h and 72h after operation. CONCLUSION: Bio-wave regulators can protect cerebral ischemic injury in rats by alleviating cerebral edema and decreasing the expression of IL-6 in the brain.