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AIM To investigate the specific pathogenesis ofO-polysaccharide (O--PS) which is on the outermembrane of lipopolysaccharides (LPS) fromShigella fi~eri.METHODS The O--PS was isolated and purifiedfrom Shigella nexneri 5 MgoT by enzymatichydrolysis and gel chromatography. Effects ofO--PS were observed by in vitro experiment,(HeLa cell Culture ), and in vivo experiment(rabbit lieal loop assay).RESULTS ID vitro and in vivo e-cP6riments withthe purified O--PS from Shigells flexnefi revealedthat the O--PS alone was toxic to Hela cells andcaused mucosal inflammation and hemorrhagicexudation in lieal loop of rabbit.DISCUSSION O--PS might b6 one of the factorscausing diarrhea and its mechanism wasdifferent from endotoxin reaction of LPS. Themolecular mechanism of O-PS need furtherstudies.
AIM To investigate the specific pathogenesis of O-polysaccharide (O - PS) which is on the outer membrane of lipopolysaccharides (LPS) from Shigella fi ~ eri.METHODS The O - PS was isolated and purified from Shigella nexneri 5 MgoT by enzymatichydrolysis and gel chromatography. Effects of O - PS were observed by in vitro experiments, (HeLa cell Culture), and in vivo experiment (rabbit lieal loop assay) .RESULTS ID vitro and in vivo e-cP6 modifications with the purified O - PS from Shigells flexnefi revealed that the O --PS alone was toxic to Hela cells andcaused mucosal inflammation and hemorrhagicexudation in lieal loop of rabbit. DISCUSSION O - PS might b6 one of the factorscausing diarrhea and its mechanism were different from endotoxin reaction of LPS. Themolecular mechanism of O-PS need furtherstudies .