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一株属于小克银汉霉属(Cunninghamellasp.)的内生真菌(编号为AL4)制成的粗诱导子可以诱发茅苍术悬浮细胞产生多种防卫反应,包括一氧化氮(NO)、过氧化氢(H2O2)迸发和挥发油合成加强。NO专一性淬灭剂cPTIO和H2O2淬灭剂过氧化氢酶(CAT)则不仅可以分别抑制AL4粗诱导子引起的茅苍术细胞的NO和H2O2迸发,还都能部分阻断AL4粗诱导子促进茅苍术细胞挥发油合成。添加NO供体硝普钠(SNP)和H2O2都可引起茅苍术细胞中挥发油积累增加,但二者效果不同。因此暗示着NO和H2O2都是介导内生真菌AL4粗诱导子促进茅苍术悬浮细胞挥发油合成的信号分子。同时添加NO的淬灭剂cPTIO和H2O2的淬灭剂CAT并不能完全抑制AL4粗诱导子引起的茅苍术细胞挥发油积累增加,这表明内生真菌AL4粗诱导子还可以通过其他方式促进茅苍术悬浮细胞挥发油合成。
A crude inducer made from an endophytic fungus (numbered AL4) belonging to the genus Cunninghamella sp. (Cunninghamella sp.) induces a variety of defense responses in suspended cells of A. lancea, including nitric oxide (NO) and hydrogen peroxide. (H2O2) Bursts and Volatile Oils Synthesize. The NO specific quencher cPTIO and the H2O2 quencher catalase (CAT) not only inhibited the bursting of NO and H2O2 in the Atractylodes lucidum induced by AL4 crude inducer, but also partially blocked the AL4 crude inducer. Promote the synthesis of volatile oil from Atractylodes lancea. The addition of NO donors sodium nitroprusside (SNP) and H2O2 all resulted in increased accumulation of volatile oil in Atractylodes lanceolata cells, but the effects were different. Therefore, it is suggested that both NO and H2O2 are signal molecules that mediate the synthesis of volatile oil from suspension cells of Atractylodes lancea induced by the crude inducer AL4 of endophytic fungi. Simultaneous addition of NO quenching agent cPTIO and H2O2 quenching agent CAT did not completely inhibit the accumulation of volatile oil in A. lancea cells induced by crude inducer of AL4, indicating that the crude inducer AL4 can also promote the suspension of Atractylodes lanceola by other means. Cellular volatile oil synthesis.