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目的 建立一个简便的研究延迟性排斥反应的猪—猴异种心脏移植模型。方法 通过流式细胞仪法监测猕猴体内天然抗体水平在出生后 0 .5～ 3月龄的变化 ,选择新生梅山猪和 2 .5月龄中国猕猴分别作为供、受体 ,建立非协调性猪—猴异种移植模型。观察移植心存活时间 ,排斥后取移植心进行电镜、病理及免疫组化检查。结果 IgM类天然抗体水平出生后缓慢上升 ,3月龄时达到 31%左右 ,而IgG类天然抗体水平始终处于低水平。移植心脏平均存活时间 36h ,电镜、病理及免疫组化显示为延迟性排斥反应。结论 选择新生梅山猪和 2 .5月龄中国猕猴建立非协调性猪—猴异种移植模型 ,可方便克服急性排斥反应 ,从而有助于延迟性排斥反应的研究。
Objective To establish a simple pig-monkey xenograft model for studying delayed rejection. Methods The levels of natural antibodies in rhesus macaques were monitored by flow cytometry from 0.5 to 3 months after birth. Neonatal Meishan pigs and 2.5-month-old Chinese macaques were used as donors and recipients to establish non-coordinating pigs - Monkey xenograft model. The survival time of the transplanted heart was observed. After exclusion, the transplanted heart was examined by electron microscopy, pathology and immunohistochemistry. Results The level of natural IgM antibody increased slowly at birth and reached about 31% at 3 months of age. However, the level of natural antibodies of IgG was always low. The average heart transplant survival time 36h, electron microscopy, pathology and immunohistochemistry showed delayed rejection. Conclusions Choosing neonatal Meishan pigs and 2.5-month-old Chinese macaques to establish a non-coordinating pig-monkey xenotransplantation model can be used to overcome the acute rejection and facilitate the study of delayed rejection.