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目的:观察复方血竭对溃疡性结肠炎(UC)实验大鼠的治疗作用及其细胞因子(CK)的调节作用。方法:将动物随机分为复方血竭组、柳氮磺胺吡啶(SASP)组、模型组、正常对照组,除正常对照组外其余3组动物分别用2,4-二硝基氯苯(DNCB)建立大鼠溃疡性结肠炎模型,给予相应治疗,镜下观察其结肠黏膜病理学变化,并测定结肠组织IL-1β,IL-4,IL-6,IL-10的水平。结果:模型组大鼠结肠黏膜糜烂、炎细胞浸润与溃疡形成情况严重,其它各组分别有不同程度的炎症修复、溃疡愈合,其中尤其以复方血竭组最为明显。复方血竭组的IL-1β,IL-6水平较模型组明显降低,IL-4,IL-10的水平明显升高。结论:复方血竭抑制了UC大鼠IL-1β,IL-6,上调了IL-4和IL-10,纠正异常的免疫功能,从而有利于炎症的消除及组织的修复。
OBJECTIVE: To observe the therapeutic effect of Compound Dragon’s Blood on experimental ulcerative colitis (UC) rats and the regulation of cytokine (CK). METHODS: Animals were randomly divided into two groups: Compound Dragon’s Blood Group, SASP group, model group, and normal control group. Except the normal control group, the other 3 groups of animals were treated with 2,4-dinitrochlorobenzene (DNCB) respectively. Rat ulcerative colitis model was established and the corresponding treatment was given. The pathological changes of colonic mucosa were observed under microscope and the levels of IL-1β, IL-4, IL-6 and IL-10 in the colon tissue were determined. RESULTS: In the model group, the colonic mucosa erosion, inflammatory cell infiltration and ulcer formation were severe. In other groups, there were different degrees of inflammatory repair and ulcer healing, among which the most obvious was the compound blood group. Compared with the model group, the levels of IL-1β and IL-6 in the compound dragon’s blood group were significantly lower, and the levels of IL-4 and IL-10 were significantly higher. CONCLUSION: DCD inhibits IL-1β and IL-6 in UC rats, upregulates IL-4 and IL-10, corrects abnormal immune function, and is beneficial to the elimination of inflammation and tissue repair.