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【目的】从养殖污泥中分离筛选优良亚硝酸盐去除菌,并对其去除条件进行研究。【方法】从养殖污泥中分离亚硝酸盐去除菌,进一步通过测定比较分离菌株对亚硝酸盐的去除率,筛选优良的亚硝酸盐去除菌,通过API ID32GN细菌鉴定系统以及16S rDNA序列分析法对其进行鉴定,并采用单因子法研究其去除亚硝酸盐的条件。【结果】从养殖污泥中分离筛选了一株优良的亚硝酸盐去除菌AQ-3,其对50 mg/L亚硝酸盐的去除率高达99.47%。菌株AQ-3被鉴定为鲍曼氏不动杆菌(Acinetobacter baumannii)(GenBank登录号:JF751054.1),其16S rDNA序列与基因库中不动杆菌属菌株的16S rDNA序列有99%?100%的同源性,而且与鲍曼氏不动杆菌KF714株(GenBank登录号:AB109775)的亲缘关系最近。菌株AQ-3去除亚硝酸盐的最适初始pH范围为7?9,最佳碳源为乙酸钠和丁二酸钠,而且随着初始菌浓度的不断增大,菌株AQ-3对亚硝酸盐的去除率显著升高;随着亚硝酸盐浓度的不断增大,菌株AQ-3对亚硝酸盐的去除率逐渐降低。【结论】在丰富亚硝酸盐去除菌种质资源的同时,为该菌在养殖水体中的实际应用提供了理论基础。
【Objective】 The objective of this study was to isolate and screen excellent nitrite-removing bacteria from cultured sludge and study the removal conditions. 【Method】 The removal of nitrite from the culture sludge was carried out. The removal rate of nitrite from the isolated strains was further compared. The bacteria were screened for excellent nitrite removal. Through API ID32GN bacterial identification system and 16S rDNA sequence analysis Its identification, and the use of single factor method to study its removal of nitrite conditions. 【Result】 An excellent strain of nitrite-removing bacteria AQ-3 was isolated and screened from cultured sludge. The removal rate of nitrite from 50 mg / L was up to 99.47%. Strain AQ-3 was identified as Acinetobacter baumannii (GenBank accession number: JF751054.1) and its 16S rDNA sequence was 99% ~ 100% with the 16S rDNA sequence of Acinetobacter strain in the gene bank. And the closest relationship with Acinetobacter baumannii KF714 strain (GenBank accession number: AB109775). Strain AQ-3 removal of nitrite optimum initial pH range of 7? 9, the optimal carbon source sodium acetate and sodium succinate, and with the initial bacterial concentration increased, strain AQ-3 of nitrite The removal rate of salt increased significantly. With the continuous increase of nitrite concentration, the removal rate of nitrite by strain AQ-3 decreased gradually. 【Conclusion】 This study provided a theoretical basis for the practical application of this bacterium in aquaculture water while enriching germplasm resources of nitrite removal.