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应用高效液相色谱(HPLC)技术,对具有不同白背飞虱抗性水平的5个水稻品种25个样品中13个特征次生物质色谱峰进行了测定,并运用主成分分析和多元回归分析,建立了抗白背飞虱种群增长的预测模型:Y=152.0762+0.7749X4-13.1806X9-14.4265X10。检验结果表明,白背飞虱种群增长指数与特征峰面积之间极显著相关(r=0.9999,P=0.0058),其中峰4、峰9、峰10对应的次生化合物可能是影响水稻对白背飞虱抗性水平的主要抗原次生化合物;抗原特征峰面积总值与白背飞虱种群增长指数具有显著的相关性,拟合的线性回归方程为:Y=82.7086-2.3476X。因此,利用高效液相色谱法测定抗原特征次生物质,可以简捷、快速地测定大田白背飞虱种群在栽培品种上的发生动态,即间接估计该品种的抗虫性。此外,采用种群生命表技术与干扰作用控制指数IIPC值评价水稻品种对白背飞虱种群消长的影响,结果显示,中感品种籼小占、粤农占、小农占I总值分别为9.280、6.030、6.603,中抗虫品种粳籼89为3.689,而感虫品种双桂I为29.789,表明中抗虫品种对白背飞虱种群的抑制作用仍十分明显,而这种抑制作用主要表现在第二世代上。
The chromatographic peaks of 13 secondary metabolites in 25 samples from 5 rice cultivars with different resistance levels to BPPH were determined by HPLC. , A prediction model for the population growth of whitebacked planthopper (WBPH) was established: Y = 152.0762 + 0.7749X4-13.1806X9-14.4265X10. The test results showed that the population growth index of whitebacked planthopper was significantly correlated with the characteristic peak area (r = 0.9999, P = 0.0058). The secondary compounds corresponding to peak 4, peak 9, The main antigenic secondary compounds were resistant to planthopper. The total area of antigenic peak area was significantly correlated with the population growth index of whitebacked planthopper. The linear regression equation was: Y = 82.7086-2.3476X. Therefore, the determination of antigen-characteristic secondary biomass by high performance liquid chromatography can quickly and easily determine the occurrence of white-backed planthopper populations in cultivars in the field, that is, indirectly estimate the insect resistance of the cultivars. In addition, the effects of rice varieties on the population decline of the whitebacked planthopper, Sogatella furcifera, were evaluated using the population life table technique and the IIPC value of disturbance effect control. The results showed that the total I-value of susceptible cultivars was 9.280,6.030 , 6.603, 3.689 for the japonica rice cultivar Zhongxian 89, and 29.789 for the susceptible cultivar Shuanggui I. The results showed that the inhibitory effect of Zhongzhuo pest on whitebackedbacked planthopper population was still very obvious, but the inhibitory effect was mainly in the second Generation