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探索谷子孕穗期对干旱环境的生理生化适应机制,筛选谷子孕穗期抗旱性鉴定的生理指标。利用抗旱性不同的20个谷子品种,在模拟干旱棚内通过孕穗期干旱胁迫,研究可溶性糖、脯氨酸、丙二醛(Malondialdehyde,MDA)等渗透势调节物质的含量和保护性酶活性的变化。孕穗期干旱胁迫使谷子叶片脯氨酸、可溶性糖和MDA含量增加,超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化氢酶(Catalase,CAT)的活性增高,而过氧化物酶(Peroxi-dase,POD)的活性则降低。相关分析表明,比较干旱胁迫与非干旱胁迫条件下可溶性糖、MDA含量和SOD活性的相对值与品种的抗旱指数相关性达到显著或极显著水平。水、旱条件下可溶性糖、MDA含量和SOD活性相对值可作为谷子孕穗期抗旱性鉴定指标,利用此3项相对值与抗旱指数所建立的回归方程:y=0.111x1-0.893x2+0.682x3,可预测谷子品种孕穗期的抗旱性。
Physiological and biochemical adaptation mechanisms of the booting stage of millet to arid environment were explored and the physiological indexes identified for drought resistance at the booting stage of millet were screened out. Using 20 millet cultivars with different drought resistance, the contents of soluble sugar, proline, malondialdehyde (MDA) and other osmoregulation substances and protective enzyme activities Variety. Drought stress at booting stage increased the contents of proline, soluble sugar and malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) activity in millet leaves, whereas peroxidase -dase, POD) activity decreased. Correlation analysis showed that the correlation between soluble sugar, MDA content and SOD activity and drought resistance index of cultivars reached a significant or extremely significant level under drought and non-drought stress. Under water and drought conditions, the relative values of soluble sugar, MDA content and SOD activity could be used as drought resistance identification index at the booting stage of millet, and the regression equation established by the three relative values and the drought resistance index was y = 0.111x1-0.893x2 + 0.682x3 , Can predict the drought resistance of the booting stage of millet varieties.