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植物名称:山杏(Prunus sibirica) 材料类别:未经层积的山杏种胚。培养条件:生长培养基以MS为基本培养基,附加BA0.1～2.0mg/L(单位下同),NAA0.1～2.0或IBA0.1～2.0;生根培养基为1/2MS附加IBA0.1～2.0。培养温度为24±1℃, 每日光照10小时,光照度1000lx。生长与分化情况:将未经层积处理的成熟种子(优系Ⅰ)用冷水浸泡24小时,去掉种皮和子叶后按不同处理接种于具有不同激素浓度的培养基。 1.种胚的萌发:未打破休眠的种胚在生长培养
Plant Name: Prunus sibirica Material Category: Non-layered apricot seed embryo. Culture conditions: Growth medium with MS as the basic medium supplemented with BA0.1 ~ 2.0mg / L (the same below), NAA0.1 ~ 2.0 or IBA0.1 ~ 2.0; rooting medium for 1 / 2MS additional IBA0. 1 ~ 2.0. Incubation temperature was 24 ± 1 ℃, daily light 10 hours, illumination 1000lx. Growth and differentiation: The mature seeds without lamination (I) were soaked in cold water for 24 hours. After removing the seed coat and cotyledons, the seeds were inoculated into culture media with different hormone concentrations according to different treatments. 1. Embryo germination: not break the dormant embryo in the growth and development