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目的:探究喜炎平注射液与顺铂联合用药是否存在协同抑制肺癌细胞的作用。方法:体外培养人肺癌H460细胞株,经喜炎平注射液和(或)顺铂作用后,采用Hoechst 33342流式细胞术检测细胞凋亡的变化程度,采用MTT法检测肺癌细胞的存活率。结果:喜炎平注射液联合顺铂能够明显抑制肺癌增殖,其抑制效果明显强于两药单用;喜炎平注射液联合顺铂能够导致肺癌细胞凋亡,其作用机制可能为活化Caspase-3、Caspase-7,激活Caspase系统,从而诱导人肺癌H460细胞株细胞凋亡。结论:喜炎平注射液和顺铂两药联用具有明显的协同作用,能有效抑制肺癌细胞增殖,并且诱导细胞凋亡,其作用机制可能与活化Caspase系统有关。
Objective: To investigate whether Xiyanping injection combined with cisplatin can inhibit the lung cancer cells synergistically. Methods: Human lung cancer cell line H460 was cultured in vitro and treated with Xiyanping injection and / or cisplatin. Hoechst 33342 flow cytometry was used to detect the changes of apoptosis. The survival rate of lung cancer cells was detected by MTT assay. Results: Xiyanping injection combined with cisplatin can significantly inhibit the proliferation of lung cancer, the inhibitory effect is significantly stronger than the two drugs alone; Xiyanping injection combined with cisplatin can cause lung cancer cell apoptosis, its mechanism may be activation of Caspase- 3, Caspase-7, activate Caspase system, thereby inducing apoptosis in human lung cancer H460 cell line. Conclusion: Xiyanping injection combined with cisplatin has obvious synergistic effect, which can effectively inhibit lung cancer cell proliferation and induce apoptosis, and its mechanism may be related to activating Caspase system.