【摘 要】
：
Background Potassium (K+) channels are important in regulating cell membrane potential and excitability. Although bronchial myocytes from asthmatic rats show a
【机 构】
：
Department of Respiratory Medicine
论文部分内容阅读
Background Potassium (K+) channels are important in regulating cell membrane potential and excitability. Although bronchial myocytes from asthmatic rats show a significant reduction in voltage-dependent delayed rectifier potassium channel (Kv) current density and higher excitability, the activity and expression of Kv in human bronchial smooth muscle cells (HBSMCs) have never been studied. The objective of this study was to investigate the effect of passive sensitization by asthmatic serum on the activity of Kv and the expression of Kv isoform Kv1.5 in HBSMCs.Methods HBSMCs were randomly divided into two groups: control group (containing 10% serum from nonatopic individuals) and sensitized group (containing 10% asthmatic serum), then cultured for 24 hours. Whole-cell patch clamp, immunofluorescence staining, reverse transcription-polymerase chain reaction and Western blot techniques were used to study the effect of passive sensitization on the activity of Kv and the expression of Kv1.5 in HBSMCs.Results The membrane potential in passively sensitized HBSMCs was significantly depolarized to -(26.7±5.2) mV compared with -(41.3±6.4) mV in the control group (P
其他文献
A novel intermolecular phosphoryl transfer from O-trimethylsilyl-N-(O, O-diisopropyl) phosphoryl serine trimethylsilyl ester to N, N-bis(trimethylsilyl) histidi
A rapid, simple and accurate method using an immunoaffinity column (IAC) and capillary electrophoresis (CE) for the analysis of the major alkaloids in opium is
Abstract Background Adiponectin, secreted by adipocytes, has been found to be associated with diabetes, obesity and some cardiovascular diseases. Obstructive s
Theimportanceofa pressure flowstudyinthediagnosticwork upof patientssufferingfrombenignprostatichyperplasia (BPH )hasbeenrecognized However,thereisstilluncertai
The density distribution and internal structure of trabecular bone are modulated by local mechanical environment.In this study,an orthotropic adaptation algorit
Background Cardiovascular diseases, especially coronary artery disease (CAD), are major causes of death in industrialized countries. Elevated concentrations of
Background There is currently considerable interest in the potential value of selective inhibitors of cyclic nucleotide phosphodiesterase 4 in the treatment of
Themaindrug resistancemechanismofgram negativebacteriais producing β lactamases Twokindsofenzymescausedrugresistancebyhydrolyzingoxyimino cephalosporinsandaztr
Previous studies have demonstrated an increase of neutrophils in lung tissues, bronchoalveolar lavage fluid (BALF), and sputum of subjects with severe, often gl
Background Successful periodontal regeneration depends on the migration, proliferation and differentiation of periodontal ligament cells in periodontal defects.