【摘 要】
：
目的 以基因工程干细胞治疗的方式来改善缺血-再灌注状况,使移植肝脏更好地耐受缺血-再灌注,以减少移植术后的并发症,延长移植器官存活期.方法 利用已建立的Wistar大鼠冷缺血
【机 构】
：
解放军第324医院普通外科,重庆,400020;成都军区昆明总医院肝胆外科,昆明,650032;解放军总医院肝胆外科,北京,100853
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目的 以基因工程干细胞治疗的方式来改善缺血-再灌注状况,使移植肝脏更好地耐受缺血-再灌注,以减少移植术后的并发症,延长移植器官存活期.方法 利用已建立的Wistar大鼠冷缺血原位肝移植(OLT)模型,术中经受体大鼠门静脉输入IL-13基因修饰的或未修饰的肝卵圆细胞(HOC)即转染组和未转染组,分别于术后1、3、7及14 d检测受体大鼠肝脏功能变化、组织学变化、胆管上皮细胞(BEC)的增殖情况、α-平滑肌肌动蛋白(α-SMA)的表达、肝组织中HO-1 mRNA的表达,并观察移植大鼠术后的存活情况.结果 转入IL-13基因的HOC对冷保存损伤的肝脏有一定的保护作用;术后7 d,肝移植组和未转染组的α-SMA表达较转染组明显(P<0.05);术后3 d,未转染组和转染组的BEC增殖指数明显低于肝移植组(P<0.05).转染组术后各时相点的HO-1 mRNA表达水平均明显高于相应时相点的其他各组的水平(P<0.05);移植术中经门静脉输入HOC对缺血性胆管损伤所诱导的BEC增殖有一定的抑制作用,对BEC具有一定的保护作用;肝移植组生存率明显低于假手术组和转染组(P<0.05).结论 IL-13的持续表达能促进术后移植肝内HO-1 mRNA的表达,这有利于对供肝的保护,有利于受体大鼠术后肝功能的恢复.促进HO-1 mRNA的表达是IL-13对BEC的具有保护作用的机理之一.
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