论文部分内容阅读
用 1 5对水稻微卫星引物 ,对 1 3个不同类型及来源的水稻基因型材料进行了简单序列重复片段长度的多态性(SSLPs)分析 ,并与不同的检测和分析方法进行了比较。聚丙烯酰胺凝胶电泳无论是放射性自显影分析还是荧光标记的自动测序仪分析都比高密度的琼脂糖电泳 (3% Meta Phor agarose)有效。利用荧光标记自动测序系统的 SSLPs分析较之传统的 3 2 P标记的聚丙烯酰胺凝胶电泳分析 ,具有以下优点 :(1 )借助于荧光染料标记的丰富内标 ,可以区别几个 bp之差的 SSLPs ;(2 )借助于不同的荧光标记可以在同一样孔里分析多个样品 ;(3)自动给出 SSL Ps片段的长度 ;(4)自动的数据输出和分析 ;(5 )避免了放射性同位素有可能造成的环境污染。因此 ,该方法具有快速、高效的特点
Simple sequence repeat fragment length polymorphism (SSLPs) analysis was performed on 15 rice genotypes with different types and origins using 15 microsatellite primers, and compared with different detection and analysis methods. Polyacrylamide gel electrophoresis was more effective than high-density agarose gel electrophoresis (3% Meta Phor agarose), either in autoradiography or fluorescence-labeled automated sequencer analysis. Compared with the traditional 3 2 P-labeled polyacrylamide gel electrophoresis analysis, the SSLPs analysis using the fluorescent label automatic sequencing system has the following advantages: (1) Differentiation between several bp can be distinguished by means of abundant internal standard labeled with fluorescent dye Of SSLPs; (2) multiple samples can be analyzed in the same wells with the help of different fluorescent labels; (3) the lengths of SSL Ps fragments are automatically given; (4) automatic data export and analysis; Radioactive isotopes may cause environmental pollution. Therefore, this method is fast and efficient