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背景:骨髓抑制是应用抗肿瘤药物治疗的主要副反应。目的:观察传统中药方琼玉膏对实验性肺癌小鼠化疗导致的骨髓抑制的干预效应。设计:随机对照试验。单位:暨南大学医学院。材料:实验于2001-03/10在暨南大学医学院中心试验室完成。选用48只健康的6～8周龄C57/BL小鼠。方法:使用癌细胞接种的方法制造小鼠肺癌模型,造模后随机均分3组,每组16只。①对照组,接种后次日用生理盐水0.2mL灌胃,同时以5μL/g体质量腹腔注射生理盐水,1次/d。②化疗组,顺氨氯铂20mg,用生理盐水稀释成0.2g/L,以5μL/g体质量腹腔注射,1次/d;同时以生理盐水0.2mL灌胃,1次/d。③联合组,除按化疗组用顺氨氯铂外,同时用琼玉膏0.2mL灌胃,1次/d。于用药后21d,检测外周血红细胞、白细胞、血小板数及骨髓有核细胞计数。主要观察指标:各组小鼠外周血红细胞计数、白细胞计数、血小板计数及骨髓有核细胞计数。结果:对照组有3只,化疗组有4只,联合组有4只未成瘤,被排除。在实验过程中对照组、化疗组各有2只死亡,联合组也有1只死亡,进入结果分析数对照组为11只、化疗组为10只、联合组为11只。①联合组及对照组的外周血红细胞、白细胞及血小板皆明显高于化疗组犤红细胞:(8.54±0.81),(8.65±0.77),(4.56±1.00)犦×1012L-1;白细胞:犤(9.04±0.60),(9.14±0.71),(3.31±0.96)犦×109L-1;血小板:犤(949.09±111.31),(955.54±87.13),(399.30±131.36)犦×109L-1,P<0.01犦。②化疗组骨髓有核细胞数明显低于联合组与对照组犤(5.30±1.12),(10.51±1.15),(14.36±1.02)犦×106,P<0.01)。而联合组与对照组相比,对照组又高于联合组(P<0.05)。结论:琼玉膏能提高肺癌小鼠化疗后外周血红细胞、白细胞、血小板及骨髓有核细胞计数,改善化疗所致的骨髓抑制状况,但尚不能完全拮抗化疗的骨髓抑制。
Background: Myelosuppression is a major side effect of antineoplastic therapy. Objective: To observe the intervention effect of traditional Chinese medicine Fang Qiongyu Gao on myelosuppression induced by chemotherapy in experimental lung cancer mice. Design: Randomized controlled trial. Unit: Jinan University School of Medicine. Materials: The experiment was performed at the Central Laboratory of Jinan University from March to October 2001. Forty eight healthy C57 / BL mice aged 6-8 weeks were selected. Methods: The mouse lung cancer model was made by inoculation of cancer cells. After model establishment, mice were randomly divided into 3 groups, 16 in each group. ① control group, the next day after inoculation saline 0.2mL gavage, while 5μL / g body weight intraperitoneal injection of saline, 1 / d. The chemotherapy group, cisplatin 20mg, diluted with physiological saline 0.2g / L, with 5μL / g body weight by intraperitoneal injection, 1 / d; at the same time with saline 0.2mL intragastric administration, 1 time / d. ③ combined group, except by chemotherapy with cisplatin, at the same time with Qiong Yu cream 0.2mL gavage, 1 / d. At 21 days after treatment, peripheral blood red blood cells, white blood cells, platelets and bone marrow nucleated cells were counted. MAIN OUTCOME MEASURES: Peripheral blood red blood cell count, white blood cell count, platelet count and bone marrow nucleated cell count in each group. Results: Three in the control group and four in the chemotherapy group, and four in the combination group did not develop tumors and were excluded. In the experiment, two of the control group and the chemotherapy group each died, and one death occurred in the combination group. The number of analysis results was 11 in the control group, 10 in the chemotherapy group and 11 in the combination group. ① The peripheral blood erythrocytes, white blood cells and platelets in the combined group and control group were significantly higher than those in the chemotherapy group (8.54 ± 0.81), (8.65 ± 0.77), (4.56 ± 1.00) × 1012L-1; leukocyte: 9.09 ± 0.60), (9.14 ± 0.71), (3.31 ± 0.96) 犦 × 109L-1, and platelets: 犤 (949.09 ± 111.31), (955.54 ± 87.13), (399.30 ± 131.36) 犦 × 109L- 0.01 犦. ② The number of bone marrow nucleated cells in chemotherapy group was significantly lower than that in combined group and control group (5.30 ± 1.12, 10.51 ± 1.15, (14.36 ± 1.02) × 106, P <0.01). The combined group compared with the control group, the control group was higher than the combined group (P <0.05). CONCLUSION: Qiong Yu Pill can improve the count of peripheral blood erythrocyte, leucocyte, platelet and bone marrow cells after chemotherapy in lung cancer mice and improve the myelosuppression caused by chemotherapy. However, it is still unable to completely antagonize the chemotherapy-induced myelosuppression.