笔者经多年实践,探索出简便实用的食用菌广谱通用型水粒菌种生产“一步法”。该方法较好地解决了传统菌种生产周期长,工艺繁,所需系列专用设备、原料、药物、用品花钱多等难题,我国城乡各地和边远山区及菇农家庭均可就地取材进行生产。其方法如下: 1.制作木粒 将阔叶树木枝条去皮,加工成长3厘米、直径约3毫米、1头呈锥形的微型木粒,煮制后盛入500毫升生理盐水瓶内,粒面覆盖1厘米厚的木屑和麸皮各50％混配的封口料,用棉塞堵封瓶口,放入太空灭菌包内灭菌3～4小时,冷却后取出。 2.接种培养 将上述经灭菌的木粒料瓶分别置“四不 After years of practice, the author has explored a “one-step approach” to produce a simple and practical broad-spectrum edible waterborne bacteria species. The method solves the difficult problem of traditional bacteria production period is long, the process is complicated, the required series of special equipment, raw materials, medicines, supplies cost more and so on. Our country urban and rural areas and remote mountainous areas and mushroom farm families can be made locally produce. The method is as follows: 1. Making wood grain The broad-leaved tree branches peeled and processed into 3 cm in diameter, about 3 mm in diameter, a tapered pyramid of wood pellets, cooked into 500 ml saline bottle, grain Cover the 1 cm thick sawdust and bran 50% mixed with the sealing material, plug the bottle stopper with a cotton tampon, put it in a sterilizing bag and sterilize for 3 to 4 hours, remove it after cooling. 2. Inoculation The above sterilized wood pellets were set "four no