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本文旨在鉴定福建省2014~2015年冬季导致病毒性胃肠炎暴发的病原体,并对病原体进行分子特征研究。收集福建省2014~2015年胃肠炎暴发期间急性病例的标本,开展诺如病毒检测并对衣壳蛋白基因片段进行分子特征分析。共检测3起暴发疫情标本31份,检出GⅡ型诺如病毒阳性标本17份。测序结果证实有13份标本为诺如病毒GⅡ.17新变异株,与2014年日本毒株Kawasaki 323同源性最高,在系统进化上形成一个新的独立的分枝,有别于近年来本地散发流行及全球暴发流行的优势基因型GⅡ.4。是福建省内首次检出诺如病毒GⅡ.17,并引起病毒性胃肠炎暴发。
The purpose of this paper is to identify the pathogens that cause viral gastroenteritis outbreaks in Fujian during the winter of 2014 ~ 2015 and to study the molecular characteristics of the pathogens. Samples of acute cases during the outbreak of gastroenteritis in Fujian Province from 2014 to 2015 were collected for detection of Norovirus and molecular characterization of the capsid protein gene fragment was performed. Thirty-one outbreaks of 31 outbreaks were detected, and 17 specimens of Gnormovirus positive were detected. Sequencing results confirmed that 13 specimens were new variants of Norovirus GⅡ.17 and had the highest homology with the Japanese strain Kawasaki 323 in 2014 and formed a new independent branch in phylogeny, Prevalence of epidemic and global outbreak epidemic genotype G Ⅱ.4. Is the first detection of Norovirus GII.17 in Fujian Province and caused an outbreak of viral gastroenteritis.