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本文对支气管哮喘患者和健康人采用双抗体夹心法ELISA测定sVCAM 1,用荧光免疫法测定血清ECP、血清总IgE(tIgE )和特异性IgE (sIgE )。结果显示 ,哮喘组的血清sVCAM 1显著高于对照组 ;两组血清ECP含量未见明显差异 ,但哮喘急性发作期的血清ECP水平明显高于缓解期患者 ;哮喘组血清总IgE几何均数显著高于对照组 ;血清sIgE分析提示蒿草花粉 (83 % )和户尘螨 (5 2 % )的sIgE不仅阳性检出率高 ,而且其血清sIgE的含量亦较高。多因素分析提示 :血清sVCAM 1与tIgE (r=0 5 0 3,P =0 0 14)、tIgE与sIgE (r=0 837,P =0 0 0 0 1)、ECP与哮喘疾病状态 (r=0 772 ,P =0 0 0 0 1)呈正相关关系。由此可见 ,支气管哮喘患者的血清sVCAM 1较正常人高 ,并且与血清tIgE的升高有关 ;血清sIgE增高会导致血清tIgE增高 ;血清ECP的含量增高与哮喘发作有关。
In this paper, sVCAM 1 was detected by double antibody sandwich ELISA in patients with asthma and healthy people, and serum ECP, serum total IgE (tIgE) and specific IgE (sIgE) were measured by fluorescence immunoassay. The results showed that the level of serum sVCAM 1 in asthma group was significantly higher than that in control group. There was no significant difference in serum ECP levels between the two groups, but serum ECP level in acute phase of asthma was significantly higher than that in remission stage. SIgE analysis showed that the sIgE of wormwood pollen (83%) and house dust mite (52%) were not only high positive, but also higher serum sIgE levels. Multivariate analysis showed that serum levels of sVCAM 1 and tIgE (r = 0 5 0 3, P = 0 0 14), tIgE and sIgE (r = 0 837, P = 0 0 0 0 1) = 0 772, P = 0 0 0 0 1) showed a positive correlation. Thus, the serum of patients with bronchial asthma sVCAM 1 higher than normal, and serum tIgE increased; serum sIgE increased will lead to elevated serum tIgE; serum levels of ECP and asthma attack.