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分别采用石油醚、氯仿、乙酸乙酯、正丁醇等有机溶剂对樟芝(Taiwanofungus camphoratus)发酵滤液进行萃取,萃取液在旋转蒸发仪上挥干,用少量的甲醇将其洗出自然挥干,进而研究樟芝发酵液不同萃取相的体外生物活性,结果表明:石油醚、氯仿、乙酸乙酯萃取物对人慢性粒细胞白血病细胞株K562的抑制作用明显,在5μg/mL(溶解于DMSO)的低浓度下即可达到60%以上(抑制率分别是74%、72%和66%),随着浓度的升高其作用高于5-Fu(抑制率68%);同样对于人前列腺癌LNCaP细胞,这三种萃取物的抑制作用也很明显,在25μg/mL下,三者的抑制率分别是72%、73%和69%,明显高于对照组5-Fu(抑制率66%);而对于RAW264.7巨噬细胞释放NO产量的刺激作用,只有正丁醇相在100、200、500μg/mL三种浓度下释放量分别达到12.12、13.75和14.32μmol/106 cells,明显高于对照组PBS(NO释放量为9.52μmol/106cells);对PC12神经细胞的修复作用,同损伤组相比,4种萃取物(除了25μg/mL浓度下正丁醇相)均有明显的功效,但是其作用浓度依赖性较小,在5μg/mL的低浓度下,几种萃取物的吸光度值分别是1.25、1.16、1.35和1.51,明显高于叠氮钠损伤组,显示了良好的修复作用。
The fermentation filtrate of Taiwanofungus camphoratus was extracted with petroleum ether, chloroform, ethyl acetate, n-butanol and other organic solvents respectively. The extract was evaporated to dryness on a rotary evaporator and washed out with a small amount of methanol The results showed that petroleum ether, chloroform and ethyl acetate extract had obvious inhibitory effect on K562 cell line, which was treated with 5μg / mL (dissolved in DMSO (Inhibition rates were 74%, 72%, and 66%, respectively), and its effect was higher than that of 5-Fu (68% inhibition) as the concentration was increased. Similarly, for human prostate The inhibitory effects of these three extracts were also obvious at 25μg / mL for LNCaP cells, 72%, 73% and 69%, respectively, which were significantly higher than that of 5-Fu in control group %). However, the release of NO from RAW264.7 macrophages was only 12.12,13.75 and 14.32μmol / 106 cells at 100,200 and 500μg / mL, respectively Higher than the control group PBS (NO release 9.52μmol / 106cells); PC12 nerve cell repair, Four extracts (except n-butanol phase at a concentration of 25 μg / mL) showed significant efficacy compared to the injury group, but their concentration dependence was less. At low concentrations of 5 μg / mL, several extracts The absorbance values were 1.25, 1.16, 1.35 and 1.51, respectively, which were significantly higher than that of sodium azide injury group and showed a good repair effect.