Human nuclear receptor hB1F is a novel member of the fushi tarazu factorⅠ subfamily of nuclear receptor superfamily. The studies about its homologous genes indicate that hB1F may play a key role in regulating the metabolic homeostasis of cholesterol. After obtaining the founder mice carrying the transgene of hb1f by microinjection, each founder was mated to normal C57 mouse and the positive F1 by PCR identification of the same founder were intercrossed within sisters and brothers to establish the transgenic mouse lineage. The results of F1, F2 and offspring of test cross identification showed that the widely expressed hb1f transgenic mouse lineage was established successfully in this study. The tissue morphology of the transgenic lineage was also analyzed preliminarily.