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目的研究HBVx和COX-2在肝细胞肝癌发病过程中的作用。方法145例HCC、78例肝炎后肝硬化及16例来自尸检的正常肝组织标本经病理复查后制成组织芯片,进行HE及免疫组织化学染色,评定各指标的染色指数。结果HCC组HB-Vx平均阳性表达指数高于肝硬化组(P<0.01)。HBVx在高、中、低分化HCC组间表达差异有统计学意义。COX-2免疫组化染色在肝硬化组表达较强;在HCC组和正常人肝细胞胞质中表达较弱。HCC组肿瘤细胞胞质中COX-2阳性表达指数低于肝硬化组(P<0.01)。COX-2在高、中、低HCC组间表达差异有统计学意义。相关性分析表明145例HCC中HBVx表达与COX-2表达存在正相关(P=0.000)。结论HBVx可通过调节COX-2的表达参与HCC发病过程。
Objective To investigate the role of HBVx and COX-2 in the pathogenesis of hepatocellular carcinoma. Methods Tissue microarrays were obtained from 145 HCC patients, 78 posthepatitic cirrhosis patients and 16 normal liver tissue samples from autopsy. The HE staining and immunohistochemical staining were performed to evaluate the staining index. Results The positive expression of HB-Vx in HCC group was higher than that in cirrhosis group (P <0.01). The expression of HBVx in high, moderate and poorly differentiated HCC group was statistically significant. COX-2 immunohistochemical staining in the cirrhosis group was strong; in the HCC group and normal liver cells in the cytoplasm was weakly expressed. The positive expression of COX-2 in cytoplasm of tumor cells in HCC group was lower than that in cirrhosis group (P <0.01). The expression of COX-2 in high, medium and low HCC groups was statistically significant. Correlation analysis showed that 145 cases of HCC HBVx expression and COX-2 expression was positively correlated (P = 0.000). Conclusion HBVx can participate in the pathogenesis of HCC by regulating the expression of COX-2.