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目的:探讨慢性进展性肾小球硬化及小管间质纤维化过程中肾组织中组织型纤溶酶原激活物(tPA)、尿激酶型纤溶酶原激活物(uPA)蛋白表达及生药水蛭对其的影响。方法:以7/8肾切除 SD 大鼠为实验动物模型,予水蛭治疗后分别检测大鼠体重、血压、血尿生化、尿纤溶酶原激活物(PA)活性及残肾组织常规病理,用免疫组化染色定性定量评价残肾 tPA、uPA 蛋白表达。结果:于7/8肾切除后,大鼠肾功能进行性丧失,表现为尿蛋白、血压、血 BuN、SCr 及血脂增高,残肾组织出现肾小球硬化和间质纤维化。免疫组化染色提示肾组织 tPA、uPA 表达下降。经水蛭治疗后,大鼠肾功能改善,血压下降,尿蛋白下降,残肾组织 tPA、uPA 蛋白表达增加。结论:水蛭治疗可通过改善7/8肾切除大鼠 PAs 系统的紊乱而干预肾纤维化病变的进展。
Objective: To investigate the expression of tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA) in renal tissue during chronic progressive glomerulosclerosis and tubulointerstitial fibrosis. Its impact. METHODS: A 7/8 nephrectomized SD rat was used as an experimental animal model. The body weight, blood pressure, hematuria, urinary plasminogen activator (PA) activity and residual renal tissue pathology were measured after treatment with leech, respectively. Immunohistochemical staining qualitatively and quantitatively evaluated the expression of tPA and uPA protein in residual kidney. RESULTS: After 7/8 nephrectomy, progressive loss of renal function was observed in urine protein, blood pressure, blood BuN, SCr, and elevated blood lipids. Glomerular sclerosis and interstitial fibrosis were observed in the residual kidney tissue. Immunohistochemical staining suggested that the expression of tPA and uPA in renal tissue decreased. After treatment with blisters, renal function improved, blood pressure decreased, urinary protein decreased, and expression of tPA and uPA protein in the residual kidney increased. CONCLUSION: The treatment of blister can interfere with the progression of renal fibrosis by improving the disorder of the PAs system in 7/8 nephrectomized rats.