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本文在以往整体动物实验和离体器官灌注证实冬虫夏草对氨基糖甙肾毒性损伤具有良好防治作用的基础上,采用肾小管细胞培养技术制作体外庆大霉素损伤模型,观察冬虫夏草对该模型细胞损伤的影响,并与国外常用的抑制庆大霉素细胞转运的保护剂氯化钙作比较。结果发现,冬虫夏草可减少庆大霉素损伤后肾小管细胞NAG酶释放(400mg/L庆大霉素时,虫草组19.17±2.01mu/L VS对照组38.83±1.55mu/L,P<0.01;800mg/L庆大霉素时,虫草组25.17±2.78mu/L vs对照组55.5±7.49mu/L,P<0.01)和乳酸脱氢酶释放,保护细胞Na~+·K~+-ATP酶。冬虫夏草作用优于氯化钙。
In this paper, in the past, the whole animal experiments and organ perfusion confirmed that Cordyceps sinensis on aminoglycoside nephrotoxicity injury has a good effect on the basis of the use of tubular cell culture in vitro gentamicin model to observe the Cordyceps sinensis on the model of cell injury , And compared with the commonly used protective agent calcium chloride that inhibits the transport of gentamicin in foreign countries. The results showed that Cordyceps Sinensis could reduce the release of NAG in tubular cells after gentamicin injury (400mg / L gentamicin, 19.17 ± 2.01mu / L vs 38.83 ± 1.55mu / L, respectively, P <0.01; 800mg / L gentamicin, 25.17 ± 2.78mu / L in Cordyceps group vs 55.5 ± 7.49mu / L in control group, P <0.01), and lactate dehydrogenase release to protect Na + K + -ATPase . Cordyceps sinensis better than calcium chloride.