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采用氯仿熏蒸法和BIOLOG检测法,分别研究了黄土高原地区弃耕地在演替早期人工添加豆科植物紫花苜蓿(Medicago sativa)、草木樨(Melilotus suaveolen)、沙打旺(Astragalus huangheensis)后土壤微生物生物量和生物多样性的变化。结果表明:添加豆科植物后,土壤微生物量碳(MBC)显著增加(除草木樨外);MBC质量分数与土壤有机碳质量分数的比率、土壤微生物在BIOILOG微平板上的平均每孔颜色变化率和碳源利用能力及其代谢多样性指数均显著提高。
Chloroform fumigation and BIOLOG test were used to study the effect of soil microorganisms on Medicago sativa, Melilotus suaveolen and Astragalus huangheensis, respectively, in abandoned farmland in the early succession stage of abandoned land in Loess Plateau. Changes in biomass and biodiversity. The results showed that after adding legumes, the soil microbial biomass carbon (MBC) increased significantly (except for clorophyte); the ratio of MBC mass fraction to soil organic carbon mass fraction, the average color change rate of soil microbes on BIOILOG microplates And carbon utilization ability and its metabolic diversity index increased significantly.