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采用AFLP(Amplified fragmentlength polymorphism)技术分析了来自四川14个不同水稻种植区和50个不同籼型杂交稻稻曲病菌(Ustilaginoidea virens)的遗传多样性。20对选择性扩增EcoRⅠ和MseⅠ引物组合稻曲病菌株可产生40～56条DNA带,75个菌株共产生192条清晰可见的DNA带型,其中多态性121条,占总数的63.02%。结果表明:不同地理来源稻曲病菌可能具有相同的遗传群体结构,也显示特异性的群体遗传特征;稻曲病菌遗传结构与寄主品种关系不大,没有表现明显的专化性互作;稻曲病菌不同的遗传群体之间菌株的致病性存在很大差异,即使同一遗传群的不同亚群或同一亚群的不同次亚群之间的菌株致病性也显著不同。
The genetic diversity of Ustilaginoidea virens from 14 different rice growing regions and 50 different indica hybrid rice varieties in Sichuan Province was analyzed by AFLP (Amplified fragment length polymorphism). 20 pairs of EcoRⅠ and MseⅠ primers selectively amplified 40-56 DNA bands in combination with the strains, and 75 strains produced 192 clearly visible DNA bands, of which 121 were polymorphic, accounting for 63.02% of the total. . The results showed that: the different geographical origin of the Y. oryzae may have the same genetic group structure, but also showed specific genetic characteristics of the population; the genetic structure of the Y. ergosovorus has little to do with the host species, did not show significant interaction; The pathogenicity of strains among different genetic groups of germs is quite different even if the pathogenicity of different strains of the same genetic group or different subgroups of the same subgroup is also significantly different.