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目的：建立成人周粘膜上皮细胞原代培养方法。方法：采用胰酶和胶原酶冷消化法消化分离手术切除的成人胃粘膜，细胞培养于含20％小牛血清的DMEM／F12培养液中。结果：细胞于种植24h后开始生长，3天长成片状，相差显微镜下可见90％以上的细胞具有上皮细胞特征。免疫组化显示90％以上的细胞上皮角蛋白染色、上皮膜抗原阳性。MTT比色法显示活细胞数于第4天达到高峰。3H-胸腺嘧啶核苷（TdR）掺入法显示细胞具有合成DNA的能力，并于第3天达到高峰。结论：本方法培养的细胞特异性高，存活时间长，为体外研究提供了较理想的模型。
Objective: To establish a method of primary culture of adult human mucosal epithelial cells. Methods: Gastric mucosa of adult patients were excised by cold digestion with trypsin and collagenase. The cells were cultured in DMEM / F12 medium containing 20% calf serum. Results: The cells began to grow after 24h of planting, grew into flakes in three days, and more than 90% of the cells showed epithelial cells under phase contrast microscope. Immunohistochemistry showed that more than 90% of cell epithelial keratin staining, epithelial membrane antigen positive. MTT colorimetric method showed that the number of viable cells reached the peak on the 4th day. The 3H-thymidine (TdR) incorporation assay showed that the cells had the ability to synthesize DNA and reached a peak on the third day. Conclusion: The method of cell culture high specificity and long survival time, in vitro studies provide an ideal model.