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AIM:TO explore whether P-glycoprotein (Pgp) and otherpumps,multidrug resistance-associated protein (MRP) andlung resistance protein (LRP),could affect tumor accumulationand efflux of ~(99m)Tc-MIBI in liver cancer.METHODS:Surgically treated 78 liver cancer patients wereincluded in this study.Before surgery,~(99m)Tc-MIBI SPECTwas performed 15 rain and 120 min after injection of 20 mCi~(99m)Tc-MIBI,respectively.Early uptake,delayed uptake (L/Nd),and washout rate (L/Nwr) of ~(99m)Tc-MIBI were obtained.Expressions of Pgp,MRP and LRP were investigated withWestern blotting and immunohistochemistry.MessengerRNA (mRNA) level of Pgp,MRP and LRP was determined byRT-PCR.RESULTS:No ~(99m)Tc-MIBI uptakes in tumor lesions of 68 of78 (87.2%) patients with hepatocellular carcinoma werefound on ~(99m)Tc-MIBI SPECT.P-gp expression was observedin tumor tissues of the patients with no uptake of ~(99m)Tc-MIBI(P<0.017).No appreciable correlation was found betweenliver cancer ~(99m)Tc-MIBI images and expression of MRP or LRPon the level of protein or mRNA.CONCLUSION:~(99m)Tc-MIBI SPECT is noninvasive,and usefulin predicting the presence of MDR1 gene-encoded Pgp inpatients with hepatocellular carcinoma.
AIM: TO explore whether P-glycoprotein (Pgp) and other pumps, multidrug resistance-associated protein (MRP) and resistance protein (LRP), could affect tumor accumulation and efflux of 99m Tc- MIBI in liver cancer. METHODS: Surgically treated 78 liver cancer patients wereincluded in this study.Before surgery, ~ (99m) Tc-MIBI SPECTwas performed 15 min and 120 min after injection of 20 mCi ~ (99m) Tc-MIBI, respectively.Early uptake, delayed uptake MRP and LRP were investigated by Western blotting and immunohistochemistry. MesRNA RNA (mRNA) level of Pgp, MRP and LRP was determined by RT-PCR, and washout rate (L / Nwr) of ~99m Tc-MIBI were obtained. Expressions of Pgp, PCR-RESULTS: No ~ (99m) Tc-MIBI uptakes in tumor lesions of 68 of 78 (87.2%) patients with hepatocellular carcinoma were found on ~ (99m) Tc- MIBI SPECT.P-gp expression was observed in tumor tissues of the patients with no uptake of ~ (99m) Tc-MIBI (P <0.017) .No appreciable correlation was found betweenliver cancer ~ (99m) Tc-MIBI images and expressi on of MRP or LRPon the level of protein or mRNA. CONCLUSION: ~ (99m) Tc-MIBI SPECT is noninvasive, and usefulin predicting the presence of MDR1 gene-encoded Pgp inpatients with hepatocellular carcinoma.