目的优化脂质体包封率测定的方法,提高脂质体包封率测定结果的准确性。方法以积雪草苷为模型药,建立HPLC法测定积雪草苷的含量;采用薄膜分散法分别制备空白脂质体与载药脂质体,利用微柱离心法分离脂质体与游离药物。分别将空白脂质体与纯化后的载药脂质体以及两者的稀释液过微柱,比较脂质体的过柱回收率。最终采用纯化脂质体的过柱回收率对所得包封率进行修正。结果在所选定的分离条件下,空白脂质体的过柱回收率为95%,纯化后的载药脂质体的过柱回收率为88%,二者存在显著性差异(P<0.05);脂质体稀释后,空白脂质体的过柱回收率无明显变化,而载药脂质体的过柱回收率则降至72.80%。结论两种方法测定脂质体的过柱回收率差异较大。采用过柱回收率修正包封率,可使测得结果更为准确。 Objective To optimize the method for determining the encapsulation efficiency of liposomes and to improve the accuracy of the determination of encapsulation efficiency of liposomes. Methods Asiaticoside was used as a model drug to establish an HPLC method for the determination of asiaticoside. Liposomes and liposomes were prepared by thin-film dispersion method. Liposomes and free drug were separated by micro-column centrifugation. . The blank liposomes and the purified drug-loaded liposomes, respectively, and the two dilutions were micro-column, liposome comparison column recovery. The final encapsulation efficiency was corrected by the column recovery of the purified liposomes. Results The column-by-column recovery of the liposome was 95% and the column-by-column recovery of the drug-loaded liposome was 88% under the conditions of the selected separation. There was a significant difference between the two ). After liposome dilution, there was no obvious change in the viability of blank liposomes, while the column recovery of drug-loaded liposomes decreased to 72.80%. Conclusion The two methods for determination of liposomes have significantly different recovery rates. The column recovery rate correction encapsulation rate, the measured results can be more accurate.