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多基因单链构象多态性(MSSCP)是一种与多基因聚合酶链反应(PCR)联合应用,一次性快速测定多基因突变的方法[1]。本研究建立了测定APC基因突变聚集区域(MCR)和Kras第12/13密码子突变的MSSCP技术。一、材料与方法1样本收集:收集...
Multi-gene single-stranded conformation polymorphism (MSSCP) is a method used in combination with multiple-gene polymerase chain reaction (PCR) to rapidly determine multiple gene mutations at a time [1]. In this study, MSSCP techniques for the detection of APC mutational aggregation regions (MCR) and K ras 12/13 codon mutations were established. I. Materials and Methods 1 Sample Collection: Collection...