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本工作的目的是测定蛇岛和盘石两地蝮蛇毒的半数致死剂量,最低溶血剂量和中枢神经毒性最小剂量以及抗蝮蛇毒血清中和力;同时也比较两地蛇毒的免疫电泳和聚丙烯酰胺凝胶园盘电泳图谱的不同,为蛇伤防治提供基础数据,并为进一步研究东北地区蝮蛇毒成分提供参考。取得结果如下: (1) 经皮下、静脉和腹腔三种不同注射途径给小白鼠注射蛇毒,其LD50是:蛇岛蝮蛇毒分别为2.5,0.62和0.94毫克/公斤,盘石蝮蛇毒分别为12.25,1.60和0.31毫克/公斤。 (2)最低溶血剂量:蛇岛蝮蛇毒为1.8微克,盘石蝮蛇毒为1.2微克。 (3)中枢神经毒最小剂量:蛇岛为4.38毫克/公斤,盘石蝮蛇毒为0.052毫克/公斤。 (4)抗蝮蛇毒血清中和力:蛇岛为27.7,盘石为57.3。 (5)免疫电泳比较结果:盘石与浙江蝮蛇毒基本相似;蛇岛与浙江蝮蛇毒差别较明显。 (6)聚丙烯酰胺凝胶园盘电泳比较:三个地区产的蝮蛇毒存在明显差别。
The purpose of this work is to determine the median lethal dose, the lowest dose of hemolysis and the minimum central neurotoxicity of snake venom and the neutralizing force of anti-snake venom serum in Snake Island and Panshi. The immune electrophoresis and polyacrylamide Gel electrophoresis map of the different patterns for the prevention and treatment of snakebites to provide the basic data, and to provide a reference for further study on the composition of snake venoms in the northeast region. The results were as follows: (1) The mice were injected subcutaneously, intravenously and intraperitoneally with three different routes of injection, the LD50 was: Snake Island viper venom were 2.5, 0.62 and 0.94 mg / kg, respectively, 1.60 and 0.31 mg / kg. (2) The lowest dose of hemolysis: Snake Island snake venom was 1.8 micrograms, snake venom was 1.2 micrograms. (3) Minimal doses of CNS: 4.38 mg / kg for Snake Island and 0.052 mg / kg for Snakeheads. (4) Anti-snake venom serum neutralization power: Snake Island is 27.7, rock is 57.3. (5) Comparison of immunoelectrophoresis: Panshi is basically similar to Agkistrodon acutus in Zhejiang Province; the difference between Snake Island and Agkistrodon acutus is obvious. (6) polyacrylamide gel electrophoresis disk comparison: viper venom produced in three regions there is a clear difference.