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以珍珠梅的腋芽茎段为外植体,探索不同杀菌时间、不同培养基、不同激素配比和浓度对珍珠梅离体培养的影响。结果表明,用0.1%HgCl2溶液,加入Tween-20,灭菌5 min处理,外植体染菌率较低,为4.8%;初代培养基以MS+6-BA0.6 mg/L的萌芽率最好,达100%,增殖倍数最高,为3.7;珍珠梅继代培养适宜的培养基为MS+6-BA0.6 mg/L+NAA0.2 mg/L,其萌芽率、增殖倍数分别为82.4%,3.7;生根培养基以1/4MS+IBA 0.2 mg/L+IAA0.2 mg/L较好,生根率、平均根数分别为85%,8,且多为实生根。
In order to explore the effects of different bactericidal time, different media, different hormone ratio and concentration on the vitro culture of pearl plum, the stem of axillary bud of pearl plum was used as explants. The results showed that with 0.1% HgCl2 solution, adding Tween-20 and sterilizing for 5 min, the infection rate of explant was low, which was 4.8%. The initial culture medium with germination rate of MS + 6-BA0.6 mg / L The best, up to 100%, the highest fold multiplication of 3.7; Pear plum subculture suitable medium MS + 6-BA0.6 mg / L + NAA0.2 mg / L, the germination rate and multiplication were 82.4%, 3.7; rooting medium with 1 / 4MS + IBA 0.2 mg / L + IAA0.2 mg / L is better, the rooting rate, the average number of roots were 85%, 8, and mostly real roots.