论文部分内容阅读
一氧化氮（ＮＯ）具有神经介质或调质的功能，广泛参与体内的生理及病理过程，具有扩张血管和神经毒性作用。体内ＮＯ系通过一氧化氮合成酶（ＮＯＳ）催化形成，并通过Ｃａ２＋／钙调素机理生成ＮＯ，因此ＮＯＳ是ＮＯ合成的关健因素。为研究脑缺血与脑组织ＮＯＳ的关系及丹参的影响，采用线栓法制成大鼠大脑中动脉缺血模型，用地高辛精标记的ＮＯＳ探针进行原位杂交。结果示缺血对照组栓塞侧皮层和尾壳核ＮＯＳ基因表达显著增多，图像分析灰阶值为：缺血侧皮层１１６．２±１．０，非缺血侧１３４．５±０．７（Ｐ＜０．０１）；缺血侧尾核壳核１２２．０±０．７，非缺血侧１３７．５±０．８（Ｐ＜０．０１）。阳性细胞数：缺血侧皮层３２６．７±８．２，非缺血侧１０７．５±９．９（Ｐ＜０．０１）；缺血侧尾壳核２６２．２±３．５，非缺血侧７２．２±９．８（Ｐ＜０．０１）。虽然丹参组缺血侧皮层和尾壳核ＮＯＳ基因表达亦显著高于非缺血侧（Ｐ＜０．０１），但显著低于缺血对照组（Ｐ均＜０．０１），而两组非缺血侧比较无显著差异。结果表明，脑缺血后脑组织ＮＯＳ基因表达显著增多，丹参能部分下调缺血所致的ＮＯＳ基因的异常表达，这可能是其治疗缺血性脑血管病的机理之一。
Nitric oxide (NO) has the function of nerve mediators or calmodulin and is widely involved in the physiological and pathological processes in the body. It has the function of dilating blood vessels and neurotoxicity. Nitric oxide (NO) is catalyzed by nitric oxide synthase (NOS) and NO is generated by Ca2 + / calmodulin mechanism. Therefore, NOS is a key factor in NO synthesis. In order to study the relationship between cerebral ischemia and brain NOS and the influence of salvia miltiorrhiza, the middle cerebral artery occlusion model of rats was made by the method of thread plugging, and the in situ hybridization was performed with digoxigenin-labeled NOS probe. The results showed that the ischemic control group showed a significant increase in NOS gene expression in the cortex and caudate putamen, the gray scale values of the ischemic cortex were 116.2 ± 1.0 in the ischemic cortex and 134.5 ± 0.7 in the non-ischemic area P <0.01). The putamen nucleus in the ischemic area was 122.0 ± 0.7 in the non-ischemic area and 137.5 ± 0.8 in the non-ischemic area (P <0.01). The number of positive cells was 326.7 ± 8.2 in ischemic cortex, 107.5 ± 9.9 in non-ischemic area (P <0.01), 262.2 ± 3.5 in ischemic caudate putamen Ischemic side 72.2 ± 9.8 (P <0.01). Although the expression of NOS gene in ischemic cortex and caudate putamen was significantly higher in Salvia miltiorrhiza than non-ischemic (P <0.01), it was significantly lower than that in ischemic control group (P <0.01) There was no significant difference in non-ischemic side. The results showed that NOS gene expression was significantly increased after cerebral ischemia, Salvia could partly down-regulate the abnormal expression of NOS gene induced by ischemia, which may be one of the mechanisms of its treatment of ischemic cerebrovascular disease.