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目的:建立赶黄草花的HPLC指纹图谱,并对赶黄草花、茎、叶不同部位的指纹图谱进行比较,寻找赶黄草不同部位质量评价与控制方法。方法:采用ODS柱,流动相乙腈-0.1%甲酸水,梯度洗脱,流速0.8 m L·min-1,柱温30℃,检测波长280 nm,进样量10μL。结果:赶黄草花共标示出15个共有峰,其中5号峰为芦丁峰,9号峰为槲皮素峰;10批赶黄草花的相似度在0.925~0.960。赶黄草花分别与茎、叶对比,花的共有峰数目最多,茎最少;茎、叶、花指纹特征峰相对含量有较大差异,花所含特征峰成分的相对含量基本高于茎和叶。结论:该方法准确可靠,重复性好,为赶黄草各部位药材质量评价与控制提供参考;为保证药材质量,建议赶黄草药材在花期采收。
OBJECTIVE: To establish HPLC fingerprinting of Phellodendron chinense Hook.f. and to compare fingerprints of different parts of Phellodendron chinense Hook.f., in order to find out the quality evaluation and control methods of the different parts of Pachnoeus chinensis. Methods: ODS column was used. The mobile phase consisted of acetonitrile-0.1% formic acid water. The flow rate was 0.8 m L · min-1 and the column temperature was 30 ℃. The detection wavelength was 280 nm and the injection volume was 10 μL. Results: Fifteen common peaks were marked on the flowers of Phellodendron amurense. Among them, No. 5 peak was rutin peak and No. 9 peak was quercetin peak. The similarity of 10 batches of Phellinus igniarius was between 0.925 and 0.960. Compared with the stem and leaves, the relative abundance of the peak of stem, leaf and flower fingerprints were significantly different, and the relative content of the characteristic peak of the flower was basically higher than that of stem and leaf . Conclusion: The method is accurate and reliable with good repeatability, which provides a reference for the quality evaluation and control of Radix phellodendron herbs. In order to ensure the quality of Radix Astragali, it is suggested that Radix Ophiopogon japonicus be harvested at anthesis.