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研究了kosmotropic型磷酸缓冲盐和硫酸钠对洋葱假单胞菌脂肪酶(Pseudomonas cepacia lipase,PCL)非水相催化性能的影响.以往磷酸缓冲盐被用来调控体系的pH值,其掺杂量对酶的催化活性无明显影响,而适量硫酸钠的掺杂则可有效提高酶在非水相的催化活性.本文研究发现,通过精确调控冻干过程,磷酸缓冲盐掺杂能够将PCL在有机相中的转酯化活性提高近10倍,达到其水相本征活性的50%,这一激活效果甚至高于硫酸钠掺杂.利用热重方法分析了盐掺杂PCL的含水量和蛋白结构,并将失重结果同其在有机相中的催化活性相关联,发现PCL在磷酸缓冲盐和硫酸钠掺杂下的催化构型与蛋白含水量及其周围盐环境具有不同的依赖关系.利用2-(4’-氨基-2’-羟基苯基)苯并恶唑作为荧光探针,研究了磷酸缓冲盐和硫酸钠掺杂的PCL悬浮于有机相时对荧光探针发射光谱的影响,发现盐掺杂酶制剂的存在能够大大增加荧光探针稳定于极性溶剂的构型含量,这可能与蛋白周围掺杂盐键和的水分子有关.如果用探针分子稳定于极性溶剂和非极性溶剂的构型比值间接表示悬浮酶制剂的极性结构,在正己烷体系中硫酸钠掺杂的PCL具有比磷酸缓冲盐掺杂的PCL大得多的极性,且酶制剂的极性大小与其非水相转酯化活性之间具有相似的变化趋势.上述研究结果表明,掺杂盐对粗PCL酶制剂的激活可能部分归因于掺杂盐键和的水分子在蛋白周围构筑的极性环境.
The effect of kosmotropic phosphate buffered saline and sodium sulfate on the non-aqueous catalytic activity of Pseudomonas cepacia lipase (PCL) was studied in the past.Phosphate buffer salts were used to control the pH value of the system, and the doping amount The catalytic activity of the enzyme had no significant effect, while the amount of sodium doping can effectively improve the catalytic activity of the enzyme in the non-aqueous phase.In this study, we found that by precisely regulating the freeze-drying process, phosphate buffer salt doping PCL in organic The trans-esterification activity in the phase increased by nearly 10-fold to reach 50% of its intrinsic activity in the aqueous phase, and this activation effect was even higher than that of sodium sulfate doping. The water content and protein structure of salt-doped PCL , And the result of weight loss was related to its catalytic activity in organic phase. It was found that the catalytic configuration of PCL under phosphate-buffered saline and sodium sulfate doping had different dependence on the protein content and the surrounding salt environment. - (4’-amino-2’-hydroxyphenyl) benzoxazole was used as a fluorescent probe to study the effect of phosphoric acid buffer salt and sodium sulfate-doped PCL on fluorescence probe emission spectra when suspended in organic phase. The presence of salt-doped enzyme preparations Can greatly increase the fluorescence probe is stabilized in polar solvent conformational content, which may be doped with salt around the protein and water molecules.If the probe molecules stabilized in polar solvent and non-polar solvent configuration ratio Indirectly indicates the polar structure of the levitation enzyme preparation, sodium sulphate-doped PCL in the n-hexane system has a much greater polarity than phosphate-buffered salt-doped PCL and the polarity of the enzyme preparation is inversely proportional to its non-aqueous phase transesterification The results of the above studies indicate that the activation of the crude PCL enzyme by the doping salt may be partly attributed to the polar environment in which the water and the water molecules are doped around the protein.