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在水温11.0～20.0℃、盐度35、pH 7.5的实验室条件下,将初始平均体质量为3.89 g的仿刺参Apostichopus japonicus幼参放养在60 L的塑料水槽中,投喂添加30、60、90 mg/kgβ-胡萝卜素和虾青素的饲料,分别记为A、B、C、D、E、F组,以不添加此物质的基础饲料作为对照(G组),每组设3个重复,饲养80 d后测定幼参的生长和几种抗氧化酶的活性。结果表明:摄食添加β-胡萝卜素的饲料时,仿刺参的特定生长率随添加量的增加而升高,A、B、C组分别比对照组提高6.67%、73.33%和100.0%,但仅C组与对照组差异显著(P<0.05);摄食添加60 mg/kg和90 mg/kg虾青素的饲料时,仿刺参的特定生长率比对照组提高113.33%和66.67%,但仅E组与对照组差异显著(P<0.05);试验组仿刺参体腔液中的总抗氧化能力(T-AOC)随饲料中β-胡萝卜素和虾青素含量的增加而升高,除A、B组外其余各试验组均显著高于对照组(P<0.05),饲料中添加虾青素的各组仿刺参体腔液的平均总抗氧化能力(12.77 U/mL)比添加β-胡萝卜素的各组平均值(8.77 U/mL)高45.61%,表明虾青素的抗氧化能力高于β-胡萝卜素;C、E、F组仿刺参体腔液中的SOD和CAT活性均显著低于对照组(P<0.05),而A、B、D组与对照组差异不显著(P>0.05);试验组仿刺参肠道蛋白酶和淀粉酶活力及体壁成分较对照组均无显著差异(P>0.05)。
Apostichopus japonicus, an initial average body weight of 3.89 g, was stocked in plastic sinks of 60 L in laboratory conditions with water temperature of 11.0-20.0 ℃, salinity of 35 and pH of 7.5, fed with 30, 60 , 90 mg / kg β-carotene and astaxanthin were respectively recorded as A, B, C, D, E and F groups, and the control group (group G) After repeated 80 days, the growth of juvenile ginseng and the activities of several antioxidant enzymes were measured. The results showed that the specific growth rate of A. ginseng increased with the addition of β-carotene, while the growth rates in groups A, B and C were increased by 6.67%, 73.33% and 100.0% respectively compared with the control group The results showed that the specific growth rate of A. ginseng increased by 113.33% and 66.67% compared with that of the control group when fed with 60 mg / kg and 90 mg / kg astaxanthin respectively Only the E group had significant difference with the control group (P <0.05). The total antioxidant capacity (T-AOC) in the body fluid of Apostichopus japonicus increased with the increase of β-carotene and astaxanthin in the feed, The average total antioxidant capacity (12.77 U / mL) in the body fluid of each group was similar to those in the control group except for the groups A and B (P <0.05) The average value of β-carotene in each group (8.77 U / mL) was 45.61%, indicating that the antioxidant capacity of astaxanthin was higher than that of β-carotene. The SOD and CAT (P <0.05). However, there was no significant difference between group A, B, D and control (P> 0.05). The protease and amylase activities and body wall contents of the experimental group were higher than those of the control group There was no significant difference between groups (P <0.05) > 0.05).