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番茄黄化曲叶病毒(Tomato yellow leaf curl virus,TYLCV)为双生病毒科(Geminiviridae)菜豆金色花叶病毒属(Begomovirus)成员,于1964年在以色列首次发现[1],目前我国已有15个省(自治区、直辖市)发现该病毒[2],TYLCV主要通过烟粉虱传播。随着分子生物学技术的不断发展,许多快速高效的检测TYLCV的方法被建立并应用于实际检测,其中以PCR技术最具实用性。研究表明PCR技术是TYLCV检测的有效手段之一[3],而实时荧光定量PCR(real-time quantitative PCR,qPCR)不仅比普通PCR更加快速、灵敏[4],而且能够对模板进行准确定量。核酸提取是现代分子生物学操作最
Tomato yellow leaf curl virus (TYLCV) is a member of the genus Begomovirus of the genus Geminiviridae. It was first discovered in Israel in 1964 [1]. At present, there are 15 Provinces (autonomous regions and municipalities) found the virus [2], TYLCV mainly transmitted by whitefly. With the continuous development of molecular biology techniques, many rapid and efficient methods for detecting TYLCV have been established and applied to the actual detection, of which PCR technology is the most practical. Studies have shown that PCR is one of the effective methods for the detection of TYLCV [3]. However, real-time quantitative PCR (qPCR) is not only faster and more sensitive than normal PCR [4], but also can accurately quantify the template. Nucleic acid extraction is the most modern molecular biology