产单核细胞李斯特菌(Lm)是重要的人类致病菌,能引起侵袭性疾病、菌血症、脑膜炎及严重的产褥热。以前所用的生物分型(噬菌体分型、多位点酶电泳分型)的分型能力有限。本文对51林Lm临床分离株(15株来源于两个流行区,36株是流行不相关菌),用传统的血清学方法、核糖分型法(RT)、脉冲凝胶电泳法(PFGE)和随机引物聚合酶链反应(PCR)法(AP-PCR)进行分型。血清学方法无法明确区分流行相关株和不相关 Listeria monocytogenes (Lm) is an important human pathogenic bacteria, can cause invasive disease, bacteremia, meningitis and severe puerperal fever. Previously used biotyping (phage typing, multilocus enzyme electrophoresis typing) typing ability is limited. In this paper, clinical isolates of 51 strains of Lm (15 strains originated from two epidemic regions and 36 strains are non-endemic) were analyzed by traditional serological methods, ribotyping method (RT), pulse gel electrophoresis (PFGE) And random primer polymerase chain reaction (PCR) method (AP-PCR) for typing. Serological methods can not be clearly distinguished between epidemic-related strains and are not relevant