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用CLC-ODS.C18柱作高效液相色谱测定小诺霉素血药浓度并做兔静注后的药代动力学研究。这一方法检测限0.2ng,最小检测血药浓度达0.1μg/ml,线性范围1～20μg/ml。小诺霉素和内标氯氮平的保留时间分别为6.8min和8.3min。方法准确、灵敏、快速,适用于小诺霉素药代动力学研究和血药浓度监测。3只新西兰兔单次静注小诺霉素10mg后,药时曲线经计算机自动拟合,符合二室开放模型。主要药动学参数为t1/2α=0.536±0.01h,t1/2β=2.59±0.98h,Vc=0.204±0.051L/kg,CL=0.18±0.026L/kg。
CLC-ODS.C18 column for high performance liquid chromatography determination of minocycline plasma concentration and rabbits intravenously after pharmacokinetic study. The detection limit of this method 0.2ng, the minimum detection plasma concentration of 0.1μg / ml, the linear range of 1 ~ 20μg / ml. The retention times of minocycline and internal standard clozapine were 6.8 min and 8.3 min, respectively. The method is accurate, sensitive and rapid, suitable for the study of minocycline pharmacokinetics and blood concentration monitoring. Three New Zealand rabbits single intravenous injection of tromycine 10mg, the drug curve automatically fitted by computer, in line with the two-compartment open model. The main pharmacokinetic parameters were t1 / 2α = 0.536 ± 0.01 h, t1 / 2β = 2.59 ± 0.98 h, Vc = 0.204 ± 0.051 L / kg and CL = 0.18 ± 0.026 L / kg.