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利用Agilent棉花寡核苷酸表达谱芯片,以2个同核异质的红麻材料P3A和P3B为试材,分析了棉花表达谱芯片应用于红麻表达谱研究的可行性。结果表明:芯片杂交扫描图像信号清晰,信噪比较高,整体背景较低,各阳性质控点信号均匀一致,空白点和阴性质控点信号低。P3A在棉花的寡核苷酸芯片上共检测到37 640个表达位点,占芯片总位点的85.93%;P3B在棉花的寡核苷酸芯片上共检测到37 629个表达位点,占芯片总位点的85.90%。34 290个探针在不育系和保持系中均具有显著的杂交信号,占总探针数43 803的78.28%。表明红麻花药cRNA与棉花表达谱芯片上寡核苷酸探针之间的远缘杂交非常有效,用棉花寡核苷酸芯片研究红麻的基因表达是可行的。
The feasibility of applying cotton expression chip to kenaf expression profile was analyzed by using Agilent cotton oligonucleotide microarrays with two isonuclear kenaf materials P3A and P3B as materials. The results showed that the chip hybridization scanning image signal is clear, the signal to noise ratio is high, the overall background is low, the signal of each positive quality control point is uniform, and the signals of the blank control point and the negative control point are low. A total of 37 640 expression sites were detected on P3A cotton oligonucleotide chips, accounting for 85.93% of the total loci. P3B detected 37 629 loci on cotton oligonucleotide chips, accounting for 85.90% of the total chip site. 34 290 probes showed significant hybridization signals in both CMS and maintainer lines, accounting for 78.28% of the total number of probes. The results showed that the hybridization between kenaf cRNA and cotton on-chip oligonucleotide probes was very effective. It is feasible to study the gene expression of kenaf using cotton oligonucleotide microarray.