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目的探讨模拟失重对体外培养大鼠心肌细胞凋亡的影响及其可能的调控机制。方法以原代培养的新生大鼠心肌细胞为研究对象,以细胞回转器模拟失重效应,通过RT-PCR检测心肌细胞凋亡相关分子c-myc mRNA在模拟失重后的变化,以western blot观察模拟失重96 h对心肌细胞c-myc和转录因子GATA-4和MEF2A蛋白表达的影响。结果与对照组相比,模拟失重可致心肌细胞凋亡相关分子c-myc mRNA及蛋白表达显著升高(P<0.05),而转录因子GATA-4和MEF2A蛋白表达显著降低(P<0.05)。结论回转器模拟失重96 h可致心肌细胞的凋亡表达增加,其机制可能与转录因子GATA-4和MEF2A表达降低有关。
Objective To investigate the effects of simulated weightlessness on cardiomyocyte apoptosis in vitro and its possible regulatory mechanism. Methods Primary cultured neonatal rat cardiomyocytes were used as experimental subjects. Cytotrotor was used to simulate the effects of weightlessness. The expression of c-myc mRNA in cardiomyocytes was detected by RT-PCR after simulated weightlessness. Effects of weightless 96 h on c-myc and GATA-4 and MEF2A protein expression in cardiomyocytes. Results Compared with the control group, the expression of c-myc mRNA and protein in cardiomyocytes was significantly increased (P <0.05) and the expressions of GATA-4 and MEF2A protein in cardiomyocytes were significantly decreased (P <0.05) . CONCLUSION: Rotavator can induce cardiomyocyte apoptosis after 96 h simulated weightlessness, which may be related to the decrease of GATA-4 and MEF2A expression.