论文部分内容阅读
目的:探讨芒果苷对脂多糖(LPS)诱导慢性炎症的抗炎作用及其机制。方法:90只SD大鼠随机分为正常组、模型组、泼尼松5 mg/kg组与芒果苷200、100、50 mg/kg组。以LPS间断尾静脉注射建立慢性炎症模型,进行大鼠全血白细胞计数,ELISA测定血清超敏C反应蛋白(hs-CRP)、肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)、可溶性细胞间粘附分子1(sICAM-1),RT-PCR检测白细胞核转录因子κB(NF-κB)基因表达。结果:与模型组比较,芒果苷200 mg/kg组全血白细胞总数与血清hs-CRP、TNF-α、IL-6、sICAM-1水平明显降低(P<0.05),白细胞NF-κB基因表达下调(P<0.05)。结论:芒果苷可显著抑制LPS诱导的慢性炎症,其作用机制与其下调白细胞NF-κB基因表达而降低炎症因子水平有关。
Objective: To investigate the anti-inflammatory effect of mangiferin on chronic inflammation induced by lipopolysaccharide (LPS) and its mechanism. Methods: 90 SD rats were randomly divided into normal group, model group, prednisone 5 mg / kg group and mangiferin 200, 100, 50 mg / kg group. The model of chronic inflammation was established by intermittent injection of LPS into the tail vein. The whole blood leukocyte count was measured. The levels of serum hs-CRP, TNF-α, IL-6 ), Soluble intercellular adhesion molecule 1 (sICAM-1), and the expression of NF-κB gene by RT-PCR. Results: Compared with the model group, the total leukocyte counts, serum hs-CRP, TNF-α, IL-6 and sICAM-1 levels in the mangiferin 200 mg / kg group were significantly lower (P < Down (P <0.05). CONCLUSION: Mangiferin can significantly inhibit LPS-induced chronic inflammation, and its mechanism may be related to downregulation of NF-κB gene expression and decrease of inflammatory cytokines.